ORIGINAL ARTICLE

CYP2E1 genotype and transaminase level of tuberculosis patients receiving fixed dose combination of antituberculosis

I Gusti Ayu Artini , I Gusti Ngurah Bagus Artana, I Gusti Made Aman, Agung Nova Mahendra

I Gusti Ayu Artini
Pharmacology Department, Medical Faculty, Udayana University, Indonesia. Email: iga_artini@yahoo.com

I Gusti Ngurah Bagus Artana
Internal Medicine Department, Medical Faculty, Udayana University, Indonesia

I Gusti Made Aman
Pharmacology Department, Medical Faculty, Udayana University, Indonesia

Agung Nova Mahendra
Pharmacology Department, Medical Faculty, Udayana University, Indonesia
Online First: August 04, 2017 | Cite this Article
Artini, I., Artana, I., Aman, I., Mahendra, A. 2017. CYP2E1 genotype and transaminase level of tuberculosis patients receiving fixed dose combination of antituberculosis. Bali Medical Journal 6(3): S70-S74. DOI:10.15562/bmj.v3i3.731


Introductions: Antituberculosis drug-induced liver injury (ATLI) had become a common serious side effect regarding anti-tuberculosis use. Isoniazid (INH) was believed as a significant factor related to ATLI incidence. A genetic factor related to INH metabolism (e.g., CYP2E1) was assumed as a major contributor of ATLI. This study aimed to investigate the genotype pattern of CYP2E1 and serum transaminase level on tuberculosis patients receiving a fixed-dose combination of anti-tuberculosis. Methods: As many as 35 tuberculosis patients attending Pulmonary Outpatient Clinic of Sanglah Hospital were included in this cross-sectional study. Identification of CYP2E1 genotype was performed with a PCR-RFLP assay using RsaI and DraI restriction enzymes. Results: This study revealed the proportion of c1/c1; c1/c2; and c2/c2 genotype of CYP2E1 on 5’-flanking region were 62.9%; 34.3%; and 2.8%, respectively; whereas the proportion of DD, CD and CC genotype of CYP2E1 on intron 6 were 60%; 28.6%; and 11.4%, respectively. The proportion of hepatotoxicity was 14.3%, while the average level of AST and ALT were 23.5±13.6 IU/L and 23.3±21.1 IU/L. There was no significant correlation between CYP2E1 genotypes and hepatotoxicity incidence. Conclusions: The dominant proportion of CYP2E1 genotype on 5’-flanking region and intron 6 are c1/c1 and DD. However, we found no significant differences between CYP2E1 genotypes and hepatotoxicity.    

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