ORIGINAL ARTICLE

CHANGES OF mRNA CASPASE-3 AFTER FIRST CYCLE OF CHEMOTHERAPY AS BIOMARKER ASSOCIATE TO CHEMOTHERAPY NEGATIVE RESPONSE IN LOCALLY ADVANCED BREAST CANCER

I K. Widiana , I. B. T. Wibawa-Manuaba, K. Siki-Kawiyana, I W. P. Sutirta-Yasa

I K. Widiana
Division of Surgical Oncology, Department of General Surgery, Faculty of Medicine Udayana University/ Sanglah General Hospital, Bali-Indonesia. Email: widray_widi@yahoo.co.id

I. B. T. Wibawa-Manuaba
Division of Surgical Oncology, Department of General Surgery, Faculty of Medicine Udayana University/ Sanglah General Hospital, Bali-Indonesia

K. Siki-Kawiyana
Division of Orthopaedic and Traumatology, Faculty of Medicine, Udayana University/ Sanglah General Hospital, Bali-Indonesia

I W. P. Sutirta-Yasa
Department of Clinical Pathology, Faculty of Medicine Udayana University/ Sanglah General Hospital, Bali-Indonesia
Online First: August 07, 2015 | Cite this Article
Widiana, I., Wibawa-Manuaba, I., Siki-Kawiyana, K., Sutirta-Yasa, I. 2015. CHANGES OF mRNA CASPASE-3 AFTER FIRST CYCLE OF CHEMOTHERAPY AS BIOMARKER ASSOCIATE TO CHEMOTHERAPY NEGATIVE RESPONSE IN LOCALLY ADVANCED BREAST CANCER. Bali Medical Journal 4(2): 53-55. DOI:10.15562/bmj.v4i2.118


Background: Problems caused by behaviour of biology molecular in locally advanced breast cancer still unpredictable. This study aims to identify the mRNA caspase-3 as a predictive biomarker associated to chemotherapy sensitivity following neoadjuvant chemotherapy (NAC) in patients with locally advanced breast cancer. Methods: Open biopsy before and core biopsy after first cycle neoadjuvant chemotherapy of 62 locally advanced breast cancer patients was analysed for mRNA caspase-3 by Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and this was correlate with response to NAC using American Joint Committee on Cancer (AJCC) creteria. Results: The total mean of mRNA caspase-3 expression before chemotherapy was 12.51±3.03 and after chemotherapy was 11.64±3.13. Negative response to chemotherapy was 44 (70.96%) and positive response was 18 (29.03%). The result of data with Phi and Cramer’s V analysed showed that decrease of mRNA caspase-3 after chemotherapy first cycle as a risk factor to chemotherapy negative response in patients with locally advanced breast cancer was significantly (p = 0.007). Conclusion: Decrease of mRNA caspase-3 after chemotherapy first cycle correlated with chemotherapy negative response in patients with lacally advanced breast cancer.
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