ORIGINAL ARTICLE

Antimicrobial susceptibility and the pattern of a biofilm-forming pair of organisms from patients treated in intensive care units in Dr. Soetomo General Hospital, Indonesia

Dewi Klarita Furtuna , Kartuti Debora, Eddy Bagus Wasito

Dewi Klarita Furtuna
Faculty of Medicine, University of Palangka Raya, Indonesia. Email: dewiklarita@med.upr.ac.id

Kartuti Debora
Department of Microbiology, Faculty of Medicine, Airlangga University/Dr. Soetomo General Hospital, Surabaya

Eddy Bagus Wasito
Department of Microbiology, Faculty of Medicine, Airlangga University/Dr. Soetomo General Hospital, Surabaya
Online First: October 30, 2018 | Cite this Article
Furtuna, D., Debora, K., Wasito, E. 2018. Antimicrobial susceptibility and the pattern of a biofilm-forming pair of organisms from patients treated in intensive care units in Dr. Soetomo General Hospital, Indonesia. Bali Medical Journal 8(1). DOI:10.15562/bmj.v8i1.1189


Background: Biofilm-associated microorganisms can cause diseases by attachment to individual cells or groups of cells on the medical device surface. The organisms may grow resistant to antibiotics. These microorganisms can be prokaryote or eukaryote organisms existing in one of two forms: sessile or planktonic. The treatment of device-associated infections with a systemic antimicrobial agent is usually ineffective.

Purpose: To find the pattern of biofilm-forming organisms and the antimicrobial susceptibility from medical devices attached to patients, so that the therapeutic management can be more accurate and useful.

Method: From 86 specimens that were analyzed, only 36 specimens showed organism growth and ability to form a biofilm. From 36 isolates analyzed for the ability to form a biofilm, only 31 isolates were in planktonic and sessile form, which were then identified and tested for antimicrobial susceptibility because the density produced was greater or equal to 0,4 (cutoff  ≥ 0,4). 

Result: Planktonic Escherichia coli from urinary and intravenous catheters matched 100% with the sessile form on the devices, as well as Klebsiella pneumoniae and Pseudomonas aeruginosa. Gram-positive cocci found was Staphylococcus aureus. Planktonic Staphylococcus aureus from urinary and intravenous catheters matched 100% with the sessile form on the devices.

Conclusion: When in planktonic form, an organism may be sterile, but the sessile form is not always sterile. The antimicrobial chosen to treat the patient should match the antimicrobial susceptibility test based on the planktonic and sessile forms of biofilm.

References

Allison DG, Gilbert P. Modification by surface association of antimicrobial susceptibility of bacterial populations. J Ind Microbiol.1995; 15:311-317

Anwar h, Dasgupta Mk, Costerton JW. Testing the susceptibility of bacteria in biofilms to antibacteria agents. Antimicrob Agents Chemother.1990;34:2043-2046

Campanac C, Pineau L, Payard A, Baziard-Mousysset G, Roques C. Interactions between biocide cationic agents and bacterial biofilms. Antimicrob Agents Chemother cause of persistent infections. Science.2002;284:1318-1322

Christensen GD, Simpson WA, Younge JA et all.Adherence of coagulase negative Staphylococcus to plastic tissue culture: quantitative model for the adherence of Staphylococci to medical device. J Clin Microbiol. 1985;22:996-1006

Donlan RM. Biofilms and Device_associated Infections.4TH Decennial International Conference Nosocomial and Healthcare-Associated Infections.2001.[cited 2016 Maret 8]. Available from: http://wwwnc.cdc.gov/eid/article/7/2/70-0277_article.

Drenkard E. Antimicrobial resistance of Pseudomonas aeruginosa bifilms. Microbes Infect. 2003;5:1213-1219

Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M. Evaluation of different detection methods of biofilm formation in the clinical isolate.2011.[cited 2015 Maret 29]. Available from : http://www.scielo.br/pdf/bjid/v15n4/v15n4a02.pdf.

Lewis K..Riddle of biofilm resistance. Antimicrob Agents Chemother.2001;45:999-1007

Mahon C.R, Lehman D.C, and Manuselis G.Diagnostic Microbiology.4 Edtion. W.B. Sauders Company.USA. 2011.

Otto Michael.Biofilms in Disease.Antibiofims Agent. From Diagnosis To Treatment and Prevention. Springer-Verlag Berlin Heidelberg.2014.

Pantanella F, Valenti P, Natalizi T, Passeri, Berlutti F.Analytical techniques to study microbial biofilm on abiotic surface: pros and cons of the main techniques currently in use. 2013. [cited 2016 Maret 8]. Available from : http://www.ncbi.nlm.nih.gov/pubmed/23435778.

Sauer K, Camper A, Ehrlich GD, Costerton JW, Davies DG. Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm. J Bacteriol. 2002;184:1140-1154

Teitzel GM, Parsek MR.Heavy metal resistance of biofilm and planktonic Pseudomonas aeruginosa. Appl Environ Microbiol. 2003;69:2313-2320

Whiteley M, Bangera MG, Bumgarner RE, Parsek MR, Teitzel GM, Lory S, Withers H, Swift S, William.Quorum sensing as an integral component of gene regulatory networks in Gram-negative bacteria. Curr Opin Microbiol. 2001;4:186-193


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