ORIGINAL ARTICLE

The Correlation between CD64 Leukocyte, Immature Granulocyte and Presepsin with Procalcitonin in Bacterial Sepsis Patient

Citra Novita, Yetti Hernaningsih , Puspa Wardhani, Anna Surgean Veterini

Citra Novita
Clinical Pathology Specialization Programe, Department of Clinical Pathology, Faculty of Medicine, Airlangga University-Dr.Soetomo Hospital, Surabaya, Indonesia

Yetti Hernaningsih
Department of Clinical Pathology, Faculty of Medicine, Airlangga University-Dr.Soetomo Hospital, Surabaya, Indonesia. Email: yettihernaningsih@gmail.com

Puspa Wardhani
Department of Clinical Pathology, Faculty of Medicine, Airlangga University-Dr.Soetomo Hospital, Surabaya, Indonesia

Anna Surgean Veterini
Department of Anesthesiology and Reanimation, Faculty of Medicine, Airlangga University-Dr.Soetomo Hospital, Surabaya, Indonesia
Online First: August 01, 2019 | Cite this Article
Novita, C., Hernaningsih, Y., Wardhani, P., Veterini, A. 2019. The Correlation between CD64 Leukocyte, Immature Granulocyte and Presepsin with Procalcitonin in Bacterial Sepsis Patient. Bali Medical Journal 8(2). DOI:10.15562/bmj.v8i2.1365


Background: Sepsis is a critical emergency that causes morbidity and mortality worldwide. The latest sepsis diagnosis is made by using quick Sepsis-Related Organ Failure Assessment (qSOFA). Cluster of Differentiation 64 (CD64) is a surface antigen leukocyte that is deregulated during infection and sepsis. The percentage of immature granulocyte (IG) could rise in patients with infection and sepsis, mainly in severe circumstances. Procalcitonin (PCT) is a calcitonin prohormone that increases in sepsis and is already known as a bacterial infection marker. Presepsin (CD14) is a glycoprotein that is known to increase in bacterial infection. This study aimed to determine the correlation of CD64 leukocyte, IG, and presepsin with PCT in bacterial sepsis patients.

Method: This cross-sectional study was performed from June to September 2018 at Dr. Soetomo Hospital. Twenty-five patients who met the qSOFA criteria with positive bacterial blood cultures were included. All samples underwent examinations of CD64 leukocyte, IG, presepsin, and PCT. The correlation of CD64 leukocytes, IG and presepsin with PCT was analyzed using Spearman correlation.

Results: The samples comprised 17 males (68.0%) and 8 females (32.0%). The mean age was 51.24 ± 14.85 years. The mean ± SD of CD64 leukocyte was 6.95 ± 2.13%, the median (min-max) of IG, presepsin and PCT was 3.67 (0.33–17.33)%, 2,641(487-20,000) pg/mL and 5.96 (0.39–181.5) ng/mL respectively. There was no correlation between CD64 leukocytes with PCT (p = 0.281). There was a significant correlation between IG and presepsin with PCT (p < 0.0001).

Conclusions: Presepsin and IG can be used as alternative bacterial sepsis markers that are supported by other examinations. CD64 leukocyte still needs to be studied further before it can be used as a bacterial sepsis marker.

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