ORIGINAL ARTICLE

The comparison of ion exchange-high performance liquid chromatography (IE-HPLC) and capillary electrophoresis (CE) for HbA1C measurement

Catur Suci Sutrisnani , Edwin Darmawan, Anik Widijanti, Sidarti Soehita

Catur Suci Sutrisnani
Metabolic Endocrinology Sub Specialist Resident at Clinical Pathology Department Faculty of Medicine Airlangga University, Surabaya. Email: 9.catursuci@gmail.com

Edwin Darmawan
Department of Clinical Pathology, Faculty of Medicine Brawijaya University/dr. Saiful Anwar Hospital, Malang

Anik Widijanti
Department of Clinical Pathology, Faculty of Medicine Brawijaya University/dr. Saiful Anwar Hospital, Malang

Sidarti Soehita
Department of Clinical Pathology, Faculty of Medicine Airlangga University, dr. Soetomo Hospital, Surabaya
Online First: April 23, 2019 | Cite this Article
Sutrisnani, C., Darmawan, E., Widijanti, A., Soehita, S. 2019. The comparison of ion exchange-high performance liquid chromatography (IE-HPLC) and capillary electrophoresis (CE) for HbA1C measurement. Bali Medical Journal 8(2). DOI:10.15562/bmj.v8i2.1379


Background:HbA1c is a glycosylated hemoglobin that routinely performed in patient with Diabetes Mellitus. There are several methods for HbA1c measurement. One of them are based on the molecular charge such as High Performance Liquid Chromatography(HPLC) and Capillary Electrophoresis (CE). Each method has its own advantages and disadvantages. Thus, the selection of HbA1c measurement method is an important thing. This study was aimed to compare HbA1c measurement with HPLC and CE method.

Methods:HbA1c measurement was done by HPLC and CE methods in 110 patient samples which have data of average blood glucose level during three months. We investigate the correlation of HbA1c results with average blood glucose level during three months,precision, methods comparison and interference including Hb varian. This study was conducted in Central Laboratory of dr. Saiful Anwar  Hospital during March–August 2018. Correlation analysis were done by Pearson analysis while precision test and methods comparison were done by Bland Altman Plot and Passing-bablok Regression analysis.

Results:There were correlation between estimated Average Glucose from HbA1c (eAGHbA1c) calculation with average blood glucose level during 3 months in both methods.From precision test we found intra-assay coefficient of variation in normal level (HbA1c ±5%) by HPLC vs CE was 1,7% vs 0.63% and in high level (HbA1c ±11%) was 0,59% vs 0,61% and inter-assay coefficient of variation by HPLC vs CE was 2,47% vs 2,17%. Method comparation results showed strong correlation on both method. The results of HbA1c in group without Hb variant by CE method was slightly lower than HPLC. In Hb variant group, CE method was slightly higher than HPLC, but it was not significant for HbA1c interpretation.

Conclusion:HbA1c measurement by HPLC and CE method are comparable and there is no significant difference in the results obtained.

Keywords


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