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The comparison of erythrocyte sedimentation rate (ESR) modify Westergren Caretium Xc-A30 and Westergren Manual in Clinical Pathology Laboratory, Sanglah General Hospital, Denpasar, Bali

Abstract

 

Background: The erythrocyte sedimentation rate (ESR) test is one of the most widely performed laboratory test to assess acute phase of inflammation, infection, autoimmune or malignancy. The reference method for ESR measurement was introduced by Westergren. This method is generally easy, un-expensive, yet, time consuming. Automatic instrument has been introduced to decrease the measurement time. Caretium XC-A30 is automatic instrument to measure ESR; it is used in Sanglah General Hospital Denpasar without previous research. Aim of this study was to assess comparison, correlation and agreement of Caretium XC- A30 to the reference method.

Methods: A total of 35 samples in consecutive sampling were taken during August 2018 at Sanglah General Hospital, Denpasar. The samples were examined for ESR with Caretium XC-A30 and manual Westergren. Comparison, correlation and agreement were tested for both methods used.

Results: The Westergren method was the reference method and Caretium XC-A30 was the tested method. Wilcoxon signed rank test showed no difference between two methods (p= 0.439). There was a very strong correlation and excellent agreement showed by Spearman coefficient correlation r= 0.989 and kappa coefficient 0.942.

Conclusion: This study showed no difference between Caretium XC-A30 and Westergren as the reference method for ESR measurement, with very strong correlation and excellent agreement. Thus, Caretium XC-A30 has a potential use in performing ESR measurement with high throughput and lesser time than manual Westergren method.

References

  1. Hoff brand AV, Moss PAH. Essential Hematology. 6th Edition. West Sussex: John Wiley & Sons Ltd. 2011: 392-396.
  2. Osei-Bimpong A, Burthem J. Supplementary Techniques for Including Blood Parasite Diagnosis. In: Dacie and Lewis Practical Hematology. Eleventh Edition. UK: Elsevier. 2011: 101-105.
  3. Jou JM, Lewis SM, Briggs C, Lee SH, Salle BDL, McFadden S. ICSH review of the Mmeasurement of the erythocyte sedimentation rate. Int J Lab Hematol. 2011 Apr;33(2):125-32
  4. Plebani M, Piva E. Erythrocyte Sedimentation Rate: use of fresh blood for quality control. Am J Clin Pathol. 2002; 117(4): 621-626.
  5. Hashemi R, Majidi A, Motamed H, Amini A, Najari F, Tabatabaey A. Erythrocyte Sedimentation Rate Measurement Using as a Rapid Alternative to the Westergren Method. Emerg (Tehran). 2015; 3(2):50-3
  6. Vennapusa B, De La Cruz L, Shah H, Michalski V, Zhang QY. Erythrocyte sedimentation rate (ESR) measured by the Streck ESR-Auto Plus is higher than with the Sediplast Westergrenmethod: a validation study. Am J Clin Pathol. 2011; 135(3): 386-390
  7. Hartono AM. Validity Test for Blood Sedimentation Test of Westergren Modification Method with a Slope Angle of 45 0 against the 1993 ICSH Reference Method. [Thesis] Bandung: Maranatha University. 2012.
  8. Vajpayee N, Graham SS, Bern S. Basic Examination of Blood and Bone Marrow. In: Henry's Clinical Diagnosis and Management by Laboratory Methods. Twenty Second. Edition. Philadelphia: Elsevier Saunders. 2011: 519-521.
  9. Clark KS, Hippel TG. Routine and Point-of-care testing in Hematology: Manual and Semiautomatic Methods. In: Hematology: Clinical Principles and Application. 4 th. Ed. Missouri: Elsevier Saunders. 2012: 172-187
  10. Setiarini W. Caretium XC-A30 ESR Analyzer User's Manual. Jakarta: PT. Setia Anugerah Medika; 2015.
  11. Fisbach FT, Dunning III MB A Manual of Laboratory and Diagnostic Test. Ninth Edition. Philadelphia: Wolters Kluwer Health | Lippincott Williams & Wilkins. 2015: 88-108.
  12. Alfadhli SM, Al-Awadhi AM. Comparison of erythrocyte sedimentation rate measurement by the automated SEDIsystem and conventional Westergrenmethod using the Bland and Altman statistical method. Med Princ Pract. 2005; 14(4): 241-4.
  13. Preet K, Anchinmane VT, Sankhe S. Evaluation of Micro-ESR Method with Westergren Method for Determination of Erythrocyte Sedimentation Rate. Int J Res Med Sci. 2018; 6(2): 628-631.
  14. Jury C, Nagai Y, Tatsumi N. Collection and Handling of Blood. In: Dacie and Lewis Practical Hematology. Eleventh Edition. UK: Elsevier. 2011: 1-7.
  15. N sifter R, Rai S, Gupta A. Essentials in Hematology and Clinical Pathology. 2nd Edition. India: Jaypee Brothers Medical Publishers (P) Ltd. 2017: 374-378.
  16. Feuerman M, Miller AR. Relationships between statistical measures of agreement: sensitivity, specificity and kappa. J Eval Clin Pract. 2008;14(5):930-3

How to Cite

Witarini Dewi, M. M., Herawati, S., Mulyantari, N. K., & Prabawa, I. P. Y. (2019). The comparison of erythrocyte sedimentation rate (ESR) modify Westergren Caretium Xc-A30 and Westergren Manual in Clinical Pathology Laboratory, Sanglah General Hospital, Denpasar, Bali. Bali Medical Journal, 8(2), 487–490. https://doi.org/10.15562/bmj.v8i2.1401

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Made Minarti Witarini Dewi
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Sianny Herawati
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Ni Kadek Mulyantari
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I Putu Yuda Prabawa
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