Skip to main content Skip to main navigation menu Skip to site footer

Red guava juice (Psidium guajava linn.) suppress TGF-β protein expression in secondary hyperuricemia mice (Mus musculus) model

  • Riska Nur Suci Ayu ,
  • Brian Wasita ,
  • Paramasari Dirgahayu ,


Background: Secondary hyperuricemia can increase the risk of renal dysfunction in the form of fibrosis and glomerulosclerosis mediated by TGF-β signalling. Non-pharmacological therapy through high dietary antioxidant intakes can help in controlling and preventing renal dysfunction by suppressing TGF-β expression. This study aimed to analyse the effect of red guava juice (Psidium Guava Linn.) on TGF-β protein expression in mice (Mus Musculus) kidneys with secondary hyperuricemia model.

Method: This was an experimental study with a post-test only design. Forty-eight mice (Mus Musculus) were choosen as samples, which were distributed into six different treatment groups. The mice were induced with potassium oxonate for 14 days and given red guava juice for 21 days. TGF-β protein expression in mice (Mus Musculus) kidneys was identified through immunohistochemistry using TGF-β antibodies. The preparation was observed in the Anatomical Pathology Laboratory, Faculty of Medicine, Universitas Sebelas Maret using the Olympus XC10 light microscope. Data were statistically analysed using the Kruskal Wallis test, followed by the Post-Hoc Mann Whitney test.

Results: The results of this study showed that the administration of red guava juice had a significant effect on TGF-β expression (p = 0.001). There was a significant difference between the negative control treatment and treatment of red guava juice at a dosage of 5 ml/kg/day (p = 0.043).

Conclusion: It can be concluded that the administration of red guava juice in mice (Mus Musculus) with secondary hyperuricemia model can suppress TGF-β expression, and therefore, can be used as non-pharmacological therapy.


  1. Doghramji P.P., Wortmann R.L. Hyperuricemia and Gout: New Concepts in Diagnosis and Management. Postgrad Med. 2012;124(6):98–109.
  2. Ozdemir A., Inci A. The effect of allopurinol on the progression of chronic kidney disease. J Nephropharmacology. 2017;6(2):138–41.
  3. Kensara O.A. Protective effect of vitamin C supplementation on oxonate-induced hyperuricemia and renal injury in rats. Int J Nutr Metab. 2013;5(June):61–6.
  4. Schnaper William H. TGF-beta signal transduction in chronic kidney disease. Front Biosci. 2009;14:2448–65.
  5. Bob F.R., Gluhovschi G., Herman D., Bozdog G., Gluhovschi C., Velciov S., et al. Histological changes and immunohistochemical markers in the assessment of glomerulosclerosis in patients with glomerulonephritis. Rom J Morphol Embryol. 2011;52(3 Suppl):1027–32.
  6. Blobe G.C., Schiemann W.P., Lodish H.F. Role of Transforming Growth Factor β in Human Disease. N Engl J Med. 2000;342(18):1350–8.
  7. Meng X.-M., Tang P.M.-K., Li J., Lan H.Y. TGF-ÃŽ2/Smad signaling in renal fibrosis. Front Physiol. 2015;6(82).
  8. Gliozzi M., Malara N., Muscoli S., Mollace V. The treatment of hyperuricemia. Int J Cardiol. 2016;213:23–7.
  9. Purwanto B. Perbedaan ekspresi TGF-β1 dan Fibrosis Interstisial pada kejadian Nefrotoksis Doxorubicin dan Nefroprotektif Pentoxifylin. J Kedokt Yars. 2009;17(2):142–9.
  10. Liu S.-F., Chang S.-Y., Lee T.-C., Chuang L.-Y., Guh J.-Y., Hung C.-Y., et al. Dioscorea alata Attenuates Renal Interstitial Cellular Fibrosis by Regulating Smad- and Epithelial-Mesenchymal Transition Signaling Pathways. PLoS One. 2012;7(11):e47482.
  11. Layal K., Perdhana I.S., Louisa M., Estuningtyas A., Soetikno V. The effects of quercetin on oxidative stress and fibrosis markers in chronic kidney disease rat model. Med J Indones. 2017;26(3):169–77.
  12. Han Y., Lu J.-S., Xu Y., Zhang L., Hong B.-F. Rutin ameliorates renal fibrosis and proteinuria in 5/6-nephrectomized rats by anti-oxidation and inhibiting activation of TGFβ1-smad signaling. Int J Clin Exp Pathol. 2015;8(5):4725.
  13. Jahagirdar K., Ghosh P., Adil S., Ziyaurrahman A. Effect of hydroalcoholics extract of Psidium Guajava Linn. on complete freund’s adjuvant induced arthritis in laboratory animals. Phaarmacologyonline. 2010;3:706–19.
  14. Lin C.-Y., Yin M.-C. Renal Protective Effects of Extracts from Guava Fruit (Psidium guajava L.) in Diabetic Mice. Plant Foods Hum Nutr. 2012;67(3):303–8.
  15. Aprillinda F.W., Paramasari D., Brian W. The effect of red guava juice (Psidium guajava Linn.) in decreasing uric acid and creatinine levels of white mice (Mus musculus) with hyperuricemia. Bali Med J. 2018;7(2).
  16. Meng X., Zhang Y., Huang X.-R., Ren G., Li J., Lan H.Y. Treatment of renal fibrosis by rebalancing TGF-β/Smad signaling with the combination of asiatic acid and naringenin. Oncotarget. 2015;6(35).
  17. Atmoko W.D., Purwanto B., Sugiarto S. Pengaruh Terapi N-Asetil Sistein terhadap Ekspresi Interleukin 17 dan Fibrosis Interstisial pada Mencit Nefritis Lupus. Biomedika. 2017;9(2):15–22.
  18. Muflikhah K. Vitamin D Aktif (1,25(OH)2D3) Mencegah Fibrosis Ginjal pada Mencit Swiss Webster Jantan dengan Unilateral Ureteral Obstruction (UUO) Kajian terhadap Kadar Transforming Growth Factor – Beta1 (TGF-β1), Fraksi Area Kolagen dan Fraksi Area Miofibroblast di Gi. Universitas Gadjah Mada; 2015.
  19. Li P.-Y., Hsu C.-C., Yin M.-C., Kuo Y.-H., Tang F.-Y., Chao C.-Y. Protective Effects of Red Guava on Inflammation and Oxidative Stress in Streptozotocin-Induced Diabetic Mice. Molecules. 2015;20(12):22341–50.
  20. Wang M., Zhao J., Zhang N., Chen J. Astilbin improves potassium oxonate-induced hyperuricemia and kidney injury through regulating oxidative stress and inflammation response in mice. Biomed Pharmacother. 2016;83:975–88.
  21. Lin C.-F., Kuo Y.-T., Chen T.-Y., Chien C.-T. Quercetin-Rich Guava (Psidium guajava) Juice in Combination with Trehalose Reduces Autophagy, Apoptosis and Pyroptosis Formation in the Kidney and Pancreas of Type II Diabetic Rats. Molecules. 2016;21(3):334.
  22. Kyselova Z. Toxicological aspects of the use of phenolic compounds in disease prevention. Interdiscip Toxicol. 2011;4(4):173–83.
  23. Yordi E., Perez E., Matos M., Villares E. Antioxidant and pro-oxidant effects of polyphenolic compounds and structure-activity relationship evidence. In: Nutrition, Well-being and Health. InTech; 2012.

How to Cite

Ayu, R. N. S., Wasita, B., & Dirgahayu, P. (2019). Red guava juice (Psidium guajava linn.) suppress TGF-β protein expression in secondary hyperuricemia mice (Mus musculus) model. Bali Medical Journal, 8(3), 915–920.




Search Panel

Riska Nur Suci Ayu
Google Scholar
BMJ Journal

Brian Wasita
Google Scholar
BMJ Journal

Paramasari Dirgahayu
Google Scholar
BMJ Journal