ORIGINAL ARTICLE

The potential application of conditioned media-mesenchymal stem cells on human oocyte maturation in assisted reproductive technology: a quasi-experimental based-study at Dr. Sardjito General Hospital, Yogyakarta, Indonesia

Dwi Haryadi , Ahmad Hamim Sadewa, Sofia Mubarika, Djaswadi Dasuki

Dwi Haryadi
Permata Hati Infertility Clinic, Departement of Obstetrics and Gynecology, Dr. Sardjito General Hospital, Yogyakarta, Indonesia. Email: dharyadiaja@gmail.com

Ahmad Hamim Sadewa
Departement of Biochemistry, Faculty of Medicine, Public Health, and Nursing, Gadjah Mada University, Yogyakarta, Indonesia

Sofia Mubarika
Departement of Histology and Cell Biology, Faculty of Medicine, Public Health, and Nursing, Gadjah Mada University, Yogyakarta, Indonesia

Djaswadi Dasuki
Permata Hati Infertility Clinic, Departement of Obstetrics and Gynecology, Dr. Sardjito General Hospital, Yogyakarta, Indonesia
Online First: December 01, 2019 | Cite this Article
Haryadi, D., Sadewa, A., Mubarika, S., Dasuki, D. 2019. The potential application of conditioned media-mesenchymal stem cells on human oocyte maturation in assisted reproductive technology: a quasi-experimental based-study at Dr. Sardjito General Hospital, Yogyakarta, Indonesia. Bali Medical Journal 8(3). DOI:10.15562/bmj.v8i3.1504


Background: PCOS typically characterized by a decreased level of GDF9 and BMP15, which contribute to follicular arrest. Recent research related to stem cells showed that conditioned media-mesenchymal stem cell (CM-MSC) might have a positive role in folliculogenesis. Therefore, this study aimed to assess the effectiveness of CM-MSC towards oocyte maturity

Method: A quasi-experimental design was used to examine the outcome of 3 different interventions, namely group A (4-hour incubation in standard media), group B (24-hour incubation in standard media), and group C (24 hours incubation in conventional media with addition of CM-MSC). Nuclear and cytoplasmic maturity, along with GDF9 and BMP15 levels were measured and analyzed.

Results: Sixty-three patients at an infertility clinic, RSUP Dr. Sardjito, Yogyakarta was recruited and a total of 292 germinal vesicles were obtained to start in vitro maturation procedure. Multivariate analysis showed group C has OR 6.9 (3,3–14,41) to obtain metaphase II oocyte than group B (p <0,0001). Infertility causes, insulin resistance, and maternal age risk are other factors that significantly influence oocyte maturity outcome (p <0.001; p = 0.005; p = 0.017). For the oocyte morphology outcome, no significant effect was obtained from the intervention (p> 0.05). Group C has higher GDF9 levels (Δ mean = 3.31) and BMP15 (Δ mean = 1.52) compared with group B (p <0.001; p = 0.006).

Conclusion: It can be concluded that 24-hour incubation in CM-MSC was effective to induce oocyte maturation. However, other factors, such as infertility causes, insulin resistance, and maternal age, should also be considered.

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