The ethyl acetate fraction of Moringa oleifera leaves effects on endothelial stress in rat sepsis model
- Tatar Sumandjar ,
- Bambang Purwanto ,
- Brian Wasita ,
- Dono Indarto ,
- Risya Cilmiaty ,
- Vitri Widyaningsih ,
Background: Sepsis is a life-threatening organ dysfunction caused by the dysregulation of the host response to infection, facilitated by inflammation and endothelial stress. Moringa oleifera leaves fractioned with ethyl acetate (MO-EA fraction) is proposed to have the ability to suppress inflammation and oxidative stress through the control of NF-kB. This study aims to investigate the effect of the MO-EA fraction in a rat sepsis model.
Material and Methods: The study is a laboratory experimental study. The research was conducted on a total of 30 rats, equally divided into five groups. One group did not receive lipopolysaccharide (LPS) induction as negative control group, but the others received LPS induction and received variable doses of MO-EA fraction (0, 10, 20, and 40 mg/kg of bodyweight). The measured outcome were serum concentration of of heparanase, CRP, malondialdehyde (MDA), and immunohistologic expression of e-selectin, NF-kB in cells, and histopathological necrosis in the aorta and kidney.
Results: MO-EA fraction significantly decrease the serum levels of HPA, MDA on day 3 and 7, and lower the CRP serum level on day 7 (p<0.05). Other variables of interest did not show significant differences.
Conclusion: The administration of MO-EA fraction of any dose significantly lower the serum level of HPA and MDA in mice sepsis models on day 3 and 7, and lower CRP on day 3 but not on day 7. However, examnationa of NF-kB and e-selectin expressions, and necrosis in kidney proximal tubule and aortic endothelial cells did not show the benefit of MO-EA fraction in preventing cell damage.