ORIGINAL ARTICLE

The effects of ethyl acetate fraction of Moringa oleifera leaves on kidney and liver function in sepsis rat model

Tatar Sumandjar , Bambang Purwanto, Riswanto Riswanto, Arif Rahman

Tatar Sumandjar
Postgraduate student in Doctorate Study Program in Public Health, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia. Email: tatar_solo@yahoo.co.id

Bambang Purwanto
Head of Doctorate Study Program in Public Health, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Riswanto Riswanto
Fellow in Internal Medicine Tropical Infection Subspecialty, Department of Internal Medicine, Dr. Moewardi General Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Arif Rahman
Resident, Department of Internal Medicine, Dr. Moewardi General Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Online First: April 01, 2020 | Cite this Article
Sumandjar, T., Purwanto, B., Riswanto, R., Rahman, A. 2020. The effects of ethyl acetate fraction of Moringa oleifera leaves on kidney and liver function in sepsis rat model. Bali Medical Journal 9(1): 271-275. DOI:10.15562/bmj.v9i1.1681


Backgrounds: Life-threatening organ dysfunction such as sepsis often happens in developing and developed countries. Ethyl acetate fraction of Moringa oleifera leaf (MO-EA fraction) is claimed by several studies to be able to control inflammation and oxidative stress.

Objective: This study aims explore the effect of MO-EA fraction in improving the liver and kidney function in sepsis rat model.

Materials and Methods: This study is an experimental research using sepsis rat model. The research was conducted on a total of 66 white Wistar rats aged 3-4 months, weighing 200-300 grams, randomly allocated into nine treatment groups, negative, and normal control groups. The treatment and the negative control group received LPS induction to reproduce the inflammation stress during a sepsis. The treatment groups receive various daily dose of MO-EA fraction starting of 10, 20, and 40 mg/kgBW. The measured outcome were serum creatinine, ureum, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SPGT).

Results:  There are statistically significant differences of the level of ureum, creatinine, SGOT, and SPGT between various treatment group receiving various dose of MO-EA fraction and negative and normal control group within seven days after LPS induction. α=0.05.

Conclusion: The serum level of ureum, creatinine, SGOT and SGPT in the sepsis rats administered with MO-EA were lower than the control group. The best MO-EA fraction is A2 which was 20 mg/kgBW/day given from 5 days before the LPS induction continuing to the seventh day after the LPS induction. MO-EA fraction supplementation may have a potential as a preventive therapy for endothelial stress in sepsis rat model.

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