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Vitamin C and ubiquinone able to maintain sperm telomers length on infertile’s men: a clinical trial at Doctor Soetomo Regional General Hospital, Surabaya, Indonesia

  • I Gusti Ngurah Pramesemara ,
  • Androniko Setiawan ,
  • Tjahjo Djojo Tanojo ,
  • Agustinus Agustinus ,
  • Judie Hartono ,


Background: Infertility is one of the clinical problems that affect medical and psychological conditions. An estimated 15% of couples in the world experience infertility and almost half occur on men. Excessive stress oxidation has been known responsible to the DNA damage and induces shortening of sperm telomere length. This study aims to determine the role of vitamin C and ubiquinone as antioxidants to maintain sperm telomers length among infertile men. 

Methods: An experimental clinical trial with a pre and post-test control group design has been conducted among 30 infertile men respondents at Doctor Soetomo Regional General Hospital, Surabaya. The respondents were divided into three groups: the combination of Vitamin C and Ubiquinone (P1), Vitamin C and Placebo (P2), and Ubiquinone and Placebo (P3) administration. All three groups were examined for pre- and post-test of sperm telomere length. Data were analyzed using SPSS version 21 for Windows.

Results: Most of the respondents were origin from Surabaya (66.7%), never been treated (66.7%), normal BMI (46.7%), non-smoking history (66.7%), non-alcohol consumption (90.0%), having 22-25 of IIEF-5 Score (60.0%), no heat exposure (90.0%), no chemical exposure (73.3%), and diagnosis with primary male infertility (90.0%). No significant difference of sperm telomere length was found among three treatment groups (p>0.05) using Post-Hoc Test. 

Conclusion: There was no significant difference in sperm telomere length of infertile men among groups. All antioxidants used in this study have a similar ability to maintain or influence sperm telomere length in infertile men.


  1. Sharlip ID, Jarow JP, Belker AM, Lipshultz LI, Sigman M, Thomas AJ, et al. Best practice policies for male infertility. Fertil Steril. 2002;77(5):873–882.
  2. Agarwal A, Sekhon LH. The role of antioxidant therapy in the treatment of male infertility. Hum Fertil (Camb). 2010;13(4):217–225.
  3. Desai N, Sabanegh E Jr, Kim T, Agarwal A. Free radical theory of aging: implications in male infertility. Urology. 2010;75(1):14–19.
  4. Thilagavathi J, Venkatesh S, Dada R. Telomere length in reproduction. Andrologia. 2013;45(5):289–304.
  5. Lombardo F, Sansone A, Romanelli F, Paoli D, Gandini L, Lenzi A. The role of antioxidant therapy in the treatment of male infertility: an overview. Asian J Androl. 2011;13(5):690–697.
  6. Süt N. Study Designs in Medicine. Balkan Med J. 2014; 31(4): 273–277.
  7. Rocca MS, Speltra E, Menegazzo M, Garolla A, Foresta C, Ferlin A. Sperm telomere length as a parameter of sperm quality in normozoospermic men. Hum Reprod. 2016;31(6):1158–1163.
  8. Montpetit AJ, Alhareeri AA, Montpetit M, Starkweather AR, Elmore LW, Filler K, et al. Telomere length: a review of methods for measurement. Nurs Res. 2014;63(4):289–299.
  9. O'Callaghan N, Dhillon V, Thomas P, Fenech M. A quantitative real-time PCR method for absolute telomere length. Biotechniques. 2008;44(6):807–809.
  10. Das P, Choudhari AR, Dhawan A, Singh R. Role of ascorbic acid in human seminal plasma against the oxidative damage to the sperms. Indian J Clin Biochem. 2009;24(3):312–315.
  11. Al-Sultani YK, Al-Katib SR, Al-Zayadi A. Effect of Vitamin C on In-Vitro Sperm Activation of Asthenozoospermic Infertile Patients. American Journal of Research Communication. 2013;1(10):40-48.
  12. Valavanidis A, Vlachogianni T. Oxidative Stress, Oxygen Free Radicals and Telomere Shortening; A Biomarker of Ageing Determines by Environmental and Genetic Factors. Science Advances on Environment, Toxicology and Ecotoxicology Issues. 2010;1-9.
  13. Cyrus A, Kabir A, Goodarzi D, Moghimi M. The effect of adjuvant vitamin C after varicocele surgery on sperm quality and quantity in infertile men: a double blind placebo controlled clinical trial. Int Braz J Urol. 2015;41(2):230–238.
  14. Akmal M, Qadri JQ, Al-Waili NS, Thangal S, Haq A, Saloom KY. Improvement in human semen quality after oral supplementation of vitamin C. J Med Food. 2006;9(3):440–442.
  15. Bhagavan HN, Chopra RK. Coenzyme Q10: absorption, tissue uptake, metabolism and pharmacokinetics. Free Radic Res. 2006;40(5):445–453.
  16. Thakur AS, Littarru GP, Funahashi I, Painkara US, Dange NS, Chauhan P. Effect of Ubiquinol Therapy on Sperm Parameters and Serum Testosterone Levels in Oligoasthenozoospermic Infertile Men. J Clin Diagn Res. 2015;9(9):BC01–BC3.
  17. Ouchi A, Nagaoka S, Mukai K. Tunneling effect in regeneration reaction of vitamin E by ubiquinol. J Phys Chem B. 2010;114(19):6601–6607.
  18. Imamovic Kumalic S, Pinter B. Review of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia. Biomed Res Int. 2014;2014:426951.

How to Cite

Pramesemara, I. G. N., Setiawan, A., Tanojo, T. D., Agustinus, A., & Hartono, J. (2020). Vitamin C and ubiquinone able to maintain sperm telomers length on infertile’s men: a clinical trial at Doctor Soetomo Regional General Hospital, Surabaya, Indonesia. Bali Medical Journal, 9(1), 360–365.




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I Gusti Ngurah Pramesemara
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Androniko Setiawan
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Tjahjo Djojo Tanojo
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Agustinus Agustinus
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Judie Hartono
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