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Investigation of Astaxanthin effect on the formation of collagen fibers for periodontal tissue repair in bacteria infected periodontitis

  • Widia Susanti ,
  • Bambang Purwanto ,
  • Risya Cilmiaty ,
  • Adi Prayitno ,


Introduction: Periodontitis is an inflammation of periodontal tissue in which the attachment-forming ligament composed of collagen fibers is damaged. Inflammation is a scenario where oxidative stress is abundant which may worsen periodontitis. Antioxidant, such as astaxanthin, is needed to promote collagen fibers formation as a sign of healing.

Objective: To explore whether astaxanthin has a desirable effect in the formation of collagen fibers in bacteria-triggered periodontitis in rats.

Methods: An animal experimental study were conducted. There were 3 groups, each consisted of 8 rats: negative control, positive control, and treated group. The treated group received oral preparation of astaxanthin 0,54 mg/kg/day for 7 days. The rats were then sacrificed, and the periodontal tissue were made into paraffin-fixed 5 µm tick slides with hematoxylin eosin staining and trichromone masson to evaluate the collagen expression. The evaluation was carried out by a pathologist.

Results: There is a significant difference of collagen expression level between the T and PC groups (p-value<0.05)

Conclusion: There is a significant difference between the groups treated with astaxanthin and the group with periodontitis disease.


  1. Nield-Gehrig JS, Willmann DE. Foundations of periodontics for the dental hygienist. Lippincott Williams & Wilkins; 2007.
  2. Abuhussein H, Bashutski JD, Dabiri D, Halubai S, Layher M, Klausner C, et al. The role of factors associated with apoptosis in assessing periodontal disease status. J Periodontol. 2014;85(8):1086–95.
  3. Smith PC, Martínez C, Martínez J, McCulloch CA. Role of fibroblast populations in periodontal wound healing and tissue remodeling. Front Physiol. 2019;10.
  4. Wong EJ. Indices Of Periodontal Disease. 1983.
  5. Halliwell B. Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol. 2006;141(2):312–22.
  6. Naguib YMA. Antioxidant activities of astaxanthin and related carotenoids. J Agric Food Chem. 2000;48(4):1150–4.
  7. Leite MF, De Lima A, Massuyama MM, Otton R. In vivo astaxanthin treatment partially prevents antioxidant alterations in dental pulp from alloxan-induced diabetic rats. Int Endod J. 2010;43(11):959–67.
  8. Zhang L, Wang H. Multiple mechanisms of anti-cancer effects exerted by astaxanthin. Mar Drugs. 2015;13(7):4310–30.
  9. Darvin ME, Fluhr JW, Schanzer S, Richter H, Patzelt A, Meinke MC, et al. Dermal carotenoid level and kinetics after topical and systemic administration of antioxidants: enrichment strategies in a controlled in vivo study. J Dermatol Sci. 2011;64(1):53–8.
  10. Draelos ZD. Nutrition and enhancing youthful-appearing skin. Clin Dermatol. 2010;28(4):400–8.
  11. Séguier S, Gogly B, Bodineau A, Godeau G, Brousse N. Is Collagen Breakdown During Periodontitis Linked to Inflammatory Cells and Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Human Gingival Tissue? J Periodontol. 2001;72(10):1398–406.
  12. Grzesik WJ, Narayanan a S, Hill C. C Ementum and P Eriodontal W Ound. Crit Rev Oral Biol Med. 2002;13(6):474–84.

How to Cite

Susanti, W., Purwanto, B., Cilmiaty, R., & Prayitno, A. (2020). Investigation of Astaxanthin effect on the formation of collagen fibers for periodontal tissue repair in bacteria infected periodontitis. Bali Medical Journal, 9(2), 493–495.




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