ORIGINAL ARTICLE

The effect of Escherichia Coli induction on superoxide dismutase (SOD) and Malondialdehyde (MDA) levels in acute rhinosinusitis white rats models

Sarwastuti Hendradewi , Bambang Purwanto, Paramasari Dirgahayu, Brian Wasita, Eti Poncorini Pamungkas

Sarwastuti Hendradewi
Department of Ear Nose Throat (ENT), Faculty of Medicine, Universitas Sebelas Maret, Dr. Moewardi General Hospital, Surakarta, Indonesia. Email: shendradewi@yahoo.com

Bambang Purwanto
Department of Internal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Dr. Moewardi General Hospital, Surakarta, Indonesia

Paramasari Dirgahayu
Department of Parasitology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta

Brian Wasita
Departement of Pathology Anatomy, Faculty of Medicine, Faculty of Medicine, Universitas Sebelas Maret, Dr. Moewardi General Hospital, Surakarta, Indonesia

Eti Poncorini Pamungkas
Department of Public Health, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Online First: August 01, 2020 | Cite this Article
Hendradewi, S., Purwanto, B., Dirgahayu, P., Wasita, B., Pamungkas, E. 2020. The effect of Escherichia Coli induction on superoxide dismutase (SOD) and Malondialdehyde (MDA) levels in acute rhinosinusitis white rats models. Bali Medical Journal 9(2): 542-545. DOI:10.15562/bmj.v9i2.1772


Background: Acute Rhinosinusitis is an inflammation of the paranasal sinuses and the nasal cavity lasting no longer than 6 weeks. One of the etiologies is the gram-negative bacteria, the lipopolysaccharide layer containing bacteria Escherichia coli. Lipopolysaccharide was the leading cause of inflammatory mechanisms, and subsequent Reactive Oxygen Species production was thought of as the agent that injure the cells thorough the oxidative stress. This study aimed to determine the effect of E-coli induction on the ratio of SOD and MDA levels in acute rhinosinusitis white rats model.

Methods: This study used an experimental post-test only control group design with white rats strain that met the inclusion and exclusion criteria divided into two groups. The study includes 8 rats as control groups and 32 rats in the treatment group. Treatment includes induction of rhinosinusitis by E-coli. SOD and MDA levels measured from retro-orbital venous blood samples were measured as marker oxidative stress on 7th, 14th,21st, and 28th days. Data were analyzed using SPSS version 22 for Windows.

Results: There were significant differences in SOD and MDA levels from day 7 to day 28 between the control and the intervention groups (p<0.001). The mean SOD levels on days 7, 14, 21, and 28, were lower than those of the control group. The MDA levels from day 7 to day 28 of the intervention group was significantly higher than that of the control group (p<0.001). Spearman Correlation test obtained a significant correlation between SOD and MDA levels (r = -0.604; p<0.001 on day 7 and r = -0.453; p< 0.003)

Conclusion: There is a significant correlation between the effect of induced E coli on SOD and MDA levels in acute rhinosinusitis white rats models.

References

Ebell MH, McKay B, Dale A, Guilbault R, Ermias Y. Accuracy of signs and symptoms for the diagnosis of acute rhinosinusitis and acute bacterial rhinosinusitis. Ann Fam Med. 2019;17(2):164–72.

Rosenfeld RM, Piccirillo JF, Chandrasekhar SS, Brook I, Ashok Kumar K, Kramper M, et al. Clinical practice guideline (update): Adult sinusitis. Otolaryngol - Head Neck Surg (United States). 2015;152:S1–39.

Fokkens WJ, Lund VJ, Hopkins C, Hellings PW, Kern R, Reitsma S, et al. Epos 2020. Off J Eur Int Rhinol Soc Confed Eur ORL-HNS. 2020;Suppl 29:1–464.

Schleimer RP. Immunopathogenesis of Chronic Rhinosinusitis and Nasal Polyposis. Annu Rev Pathol Mech Dis. 2017;12(1):331–57.

Scheckenbach K, Wagenmann M. Cytokine Patterns and Endotypes in Acute and Chronic Rhinosinusitis. Curr Allergy Asthma Rep. 2016;16(1):1–8.

Cho JS, Kang JH, Um JY, Han IH, Park IH, Heung H, et al. Lipopolysaccharide induces pro-inflammatory cytokines and mmp production via TLR4 in nasal polyp-derived fibroblast and organ culture. PLoS One. 2014;9(11):1–11.

Wang S, Zhang H, Xi Z, Huang J, Nie J, Zhou B, et al. Establishment of a mouse model of lipopolysaccharide-induced neutrophilic nasal polyps. Exp Ther Med. 2017;14(6):5275–82.

Cho DY, Nayak JV, Bravo DT, Le W, Nguyen A, Edward JA, et al. expression of dual oxidases and secreted cytokines in chronic rhinosinusitis. Int Forum Allergy Rhinol. 2013;3(5):376–83.

Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive oxygen species in inflammation and tissue injury. Antioxid Redox Signal. 2013/10/22. 2014 Mar;20(7):1126–67.

Shi JB, Fu QL, Zhang H, Cheng L, Wang YJ, Zhu DD, et al. Epidemiology of chronic rhinosinusitis: Results from a cross-sectional survey in seven Chinese cities. Allergy Eur J Allergy Clin Immunol. 2015;70(5):533–9.

Phan NT, Cabot PJ, Wallwork BD, Cervin AU, Panizza BJ. Cellular and molecular mechanisms of chronic rhinosinusitis and potential therapeutic strategies: Review on cytokines, nuclear factor kappa B and transforming growth factor beta. J Laryngol Otol. 2015;129(S3):S2–7.

Guo Z, Hong Z, Dong W, Deng C, Zhao R, Xu J, et al. PM2.5-induced oxidative stress and mitochondrial damage in the nasal mucosa of rats. Int J Environ Res Public Health. 2017;14(2).

Al-Sayed AA, Agu RU, Massoud E. Models for the study of nasal and sinus physiology in health and disease: A review of the literature. Laryngoscope Investig Otolaryngol. 2017;2(6):398–409.

Afonso V, Champy R, Mitrovic D, Collin P, Lomri A. Reactive oxygen species and superoxide dismutases: Role in joint diseases. Jt Bone Spine. 2007;74(4):324–9.

Chen Y, Luan L, Wang C, Song M, Zhao Y, Yao Y, et al. Dexmedetomidine protects against lipopolysaccharide-induced early acute kidney injury by inhibiting the iNOS/NO signaling pathway in rats. Nitric Oxide - Biol Chem [Internet]. 2019;85(January):1–9. Available from: https://doi.org/10.1016/j.niox.2019.01.009

Tasdemir C, Tasdemir S, Vardi N, Ates B, Onal Y, Erdogan S, et al. Evaluation of the effects of ozone therapy on escherichia coli-induced cytitis in rat. Ir J Med Sci. 2013;182(4):557–63.

Islam MS, Miao L, Yu H, Han Z, Sun H. Ethanol extract of Illicium henryi attenuates LPS-induced acute kidney injury in mice via regulating inflammation and oxidative stress. Nutrients. 2019;11(6).


Article Views      : 0
PDF Downloads : 0