ORIGINAL ARTICLE

Angiogenesis in Ischemic Stroke Patients Aged 30 - 80 years at Gatot Subroto Army Central Hospital (RSPAD) Jakarta

Andri Hidayat , Mansyur Arief, Andi Wijaya, Suryani As’ad

Andri Hidayat
Postgraduate Program in Clinical Biochemistry, Hasanuddin University, Indonesia. Email: andri.hidayat@prodia.co.id

Mansyur Arief
Prodia Clinical Laboratory, Indonesia

Andi Wijaya
Prodia Clinical Laboratory, Indonesia

Suryani As’ad
Prodia Clinical Laboratory, Indonesia
Online First: May 31, 2016 | Cite this Article
Hidayat, A., Arief, M., Wijaya, A., As’ad, S. 2016. Angiogenesis in Ischemic Stroke Patients Aged 30 - 80 years at Gatot Subroto Army Central Hospital (RSPAD) Jakarta. Bali Medical Journal 5(3): 367-370. DOI:10.15562/bmj.v5i3.202


Background: Angiogenesis, is one of the neurorepair process which plays an important role after ischemic stroke incident. vascular-endothelial growth factor (VEGF), Angiopoietin-1 (ANG1) and fibroblast growth factor (FGF) are the angiogenic factors involved in the process of angiogenesis. In this study we observed VEGF, ANG1 and FGF concentration to represent the angiogenesis process occurred in ischemic stroke patients with different onset time. Methods: This was a cross sectional study involving 63 ischemic stroke subjects aged 30 – 80 years old from The Central Hospital of the Army (RSPAD) Gatot Subroto Jakarta. Subjects were divided into 3 groups due to stroke onset time.: < than 7 days (Group A: 11 subjects), onset 7 – 30 days (Group B: 26 subjects) and > 30 days (Group C: 26 Subjects). VEGF, FGF and ANG1 serum levels were measured using multiplex method with luminex Magpix instrument. Results: VEGF, ANG1, and FGF were not significantly different between all groups. We did not find any significant correlation in all groups except FGF with ANG1 in group C. VEGF and ANG1 levels found to be highest in group B, and FGF levels in group A. Conclusions: VEGF, FGF and ANG1 increased soon after ischemic injury. FGF immediately increased in first week after onset and then decreased. VEGF and ANG1 levels reach their peak levels between 7-30 days after injury, showed that the optimal process of angiogenesis occurs in this period.  There is no significant correlation between VEGF, FGF and ANG1 in all groups but we found a correlation between FGF and ANG1 in subjects with onset > 30 days after injury.

References

Jablonska A, Lukomska B. Stroke induced brain changes: Implications for stem cell transplantation. Acta Neurobiol Exp (Wars). 2011;71(1):74-85.

Balitbangkes. Riset Kesehatan Dasar. In: RI BPdPK, editor.: Departemen Kesehatan RI; 2013.

Liman TG, Endres M. New vessels after stroke: postischemic neovascularization and regeneration. Cerebrovasc Dis. 2012;33(5):492-9.

Font MA, Arboix A, Krupinski J. Angiogenesis, neurogenesis and neuroplasticity in ischemic stroke. Curr Cardiol Rev. 2010;6(3):238-44.

Krupinski J, Kaluza J, Kumar P, Kumar S, Wang JM. Role of angiogenesis in patients with cerebral ischemic stroke. Stroke. 1994;25(9):1794-8.

Hayashi T, Noshita N, Sugawara T, Chan PH. Temporal profile of angiogenesis and expression of related genes in the brain after ischemia. J Cereb Blood Flow Metab. 2003;23(2):166-80.

Ergul A, Alhusban A, Fagan SC. Angiogenesis: a harmonized target for recovery after stroke. Stroke. 2012;43(8):2270-4.

MA F, A A, J K. Angiogenesis, Neurogenesis and Neuroplasticity in Ischemic Stroke. Current Cardiology Reviews. 2010;6.

Madri JA. Modeling the neurovascular niche: implications for recovery from CNS injury. J Physiol Pharmacol. 2009;60 Suppl 4:95-104.

Pugh CW, Ratcliffe PJ. Regulation of angiogenesis by hypoxia: role of the HIF system. Nat Med. 2003;9(6):677-84.

Talwar T, Srivastava MV. Role of vascular endothelial growth factor and other growth factors in post-stroke recovery. Ann Indian Acad Neurol. 2014;17(1):1-6.

Slevin M, Kumar P, Gaffney J, Kumar S, Krupinski J. Can angiogenesis be exploited to improve stroke outcome? Mechanisms and therapeutic potential. Clin Sci (Lond). 2006;111(3):171-83.

Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med. 2003;9(6):669-76.

Baburamani AA, Ek CJ, Walker DW, Castillo-Melendez M. Vulnerability of the developing brain to hypoxic-ischemic damage: contribution of the cerebral vasculature to injury and repair? Front Physiol. 2012;3:424.

Jin K, Zhu Y, Sun Y, Mao XO, Xie L, Greenberg DA. Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo. Proc Natl Acad Sci U S A. 2002;99(18):11946-50.

TM H, AJ M, PJ B. Vascular Endothelial Growth Factor and Angiopoietins in Neurovascular. Current Neurovascular Research. 2008(5):10.

Saharinen P, Alitalo K. The yin, the yang, and the angiopoietin-1. J Clin Invest. 2011;121(6):2157-9.

Ohab JJ, Fleming S, Blesch A, Carmichael ST. A neurovascular niche for neurogenesis after stroke. J Neurosci. 2006;26(50):13007-16.

Conte C, Riant E, Toutain C, Pujol F, Arnal JF, Lenfant F, et al. FGF2 translationally induced by hypoxia is involved in negative and positive feedback loops with HIF-1alpha. PLoS One. 2008;3(8):e3078.

Lanfranconi S, Locatelli F, Corti S, Candelise L, Comi GP, Baron PL, et al. Growth factors in ischemic stroke. J Cell Mol Med. 2011;15(8):1645-87.

Bouzaffour M, Dufourcq P, Lecaudey V, Haas P, Vriz S. Fgf and Sdf-1 pathways interact during zebrafish fin regeneration. PLoS One. 2009;4(6):e5824.

Chen J, Zhang C, Jiang H, Li Y, Zhang L, Robin A, et al. Atorvastatin induction of VEGF and BDNF promotes brain plasticity after stroke in mice. J Cereb Blood Flow Metab. 2005;25(2):281-90.

Sahara M, Sata M, Morita T, Nakajima T, Hirata Y, Nagai R. A phosphodiesterase-5 inhibitor vardenafil enhances angiogenesis through a protein kinase G-dependent hypoxia-inducible factor-1/vascular endothelial growth factor pathway. Arterioscler Thromb Vasc Biol. 2010;30(7):1315-24.

Zhang R, Wang L, Zhang L, Chen J, Zhu Z, Zhang Z, et al. Nitric oxide enhances angiogenesis via the synthesis of vascular endothelial growth factor and cGMP after stroke in the rat. Circ Res. 2003;92(3):308-13.

Slevin M, Krupinski J, Slowik A, Kumar P, Szczudlik A, Gaffney J. Serial measurement of vascular endothelial growth factor and transforming growth factor-beta1 in serum of patients with acute ischemic stroke. Stroke. 2000;31(8):1863-70.


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