The effect of Myrmecodia pendans extract toward gastric histology in white rats treated with toxic dose of gentamicin: a preliminary report

I Made Bagus Januar Bahari Putra, Ketut Budiasa, I Made Merdana, Anak Agung Ayu Mirah Adi

I Made Bagus Januar Bahari Putra
Undergraduate Student, Faculty of Veterinary Medicine, Universitas Udayana, Bali, Indonesia

Ketut Budiasa
Pharmacology Department, Faculty of Veterinary Medicine, Universitas Udayana, Bali, Indonesia

I Made Merdana
Pharmacology and Pharmacy Department, Faculty of Veterinary Medicine, Universitas Udayana, Denpasar, Bali

Anak Agung Ayu Mirah Adi
Pathology Department, Faculty of Veterinary Medicine, Universitas Udayana, Denpasar, Bali. Email: aaa_mirahadi@unud.ac.id
Online First: December 26, 2020 | Cite this Article
Putra, I., Budiasa, K., Merdana, I., Adi, A. 2020. The effect of Myrmecodia pendans extract toward gastric histology in white rats treated with toxic dose of gentamicin: a preliminary report. Bali Medical Journal 9(3): 924-928. DOI:10.15562/bmj.v9i3.2079

Introduction: Myrmecodia pendans, or traditionally known as ant nest fruit, is one of popular traditional medicines in Indonesia with a wide range of effects such as antidiabetic, anticancer, and free-radical scavenging agents. Despite of the extensive research, reports regarding its toxicity are scarce. Gentamicin is also one of popular and widely used anti-biotic but it is associated with a wide range of toxicity, especially if administered at high dose. Therefore, this study aimed to evaluate the effect of Myrmecodia pendans extract in the mice what received toxic dose of gentamicin.

Method: 24 male white rats were used and randomly divided into 4 groups: P0 (negative control), P1 (100 mg/kgBW intraperitoneal gentamicin), P2 (gentamicyn + 250 mg/kgBW myrmercodia extract), P3 (250 mg/kgBW myrmercodia extract which administered for 7 days and followed by gentamicin). The treatment was given for 10 days and all mice were sacrificed at the end of the study. The stomach was taken from each mouse and examined pathologically using Hematoxylin-Eosin (HE) staining.

Results: The necrosis was found in all groups that received gentamicin (P1-P3) with P2 had the most severe necrosis. Hemorrhage was only found in group that received Myrmecodia extract (P2 and P3) and, again, P2 had higher score of hemorrhage compared to P3.

Conclusion: The administration of Myrmecodia extract at 250 mg/kgBW together with toxic dose of gentamicin exacerbate the gastric tissue damage in Wistar rats.


Soeksmanto A, Subroto MA, Wijaya H, Simanjuntak P. Anticancer activity test for extracts of Sarang semut plant (Myrmecodya pendens) to HeLa and MCM-B2 cells. Pakistan J Biol Sci PJBS [Internet]. 2010;13(3):148–51. Available from: http://europepmc.org/abstract/MED/20437705

Engida AM, Faika S, Nguyen-Thi BT, Ju Y-H. Analysis of major antioxidants from extracts of Myrmecodia pendans by UV/visible spectrophotometer, liquid chromatography/tandem mass spectrometry, and high-performance liquid chromatography/UV techniques. J food drug Anal. 2015 Jun;23(2):303–9.

Agatonovic-Kustrin S, Morton DW, Adam A, Mizaton HH, Zakaria H. High-performance thin-layer chromatographic methods in the evaluation of the antioxidant and anti-hyperglycemic activity of Myrmecodia platytyrea as a promising opportunity in diabetes treatment. J Chromatogr A. 2017 Dec;1530:192–6.

Achmad H, Supriatno, Singgih MF, Hendrastuti H. Akt Signal Transduction Pathways and Nuclear Factor-kappa B (NF-κB) Transcription as a Molecular Target of Oral Tongue Squamous Cell Carcinoma (SP-C1) Using Papua’s Anthill Plant ( Myrmecodia pendans ). Pakistan J Biol Sci PJBS. 2016;19(8–9):323–30.

Kurnia D, Apriyanti E, Soraya C, Satari MH. Antibacterial Flavonoids Against Oral Bacteria of Enterococcus Faecalis ATCC 29212 from Sarang Semut (Myrmecodia pendans) and Its Inhibitor Activity Against Enzyme MurA. Curr Drug Discov Technol. 2019;16(3):290–6.

Gonzalez LS 3rd, Spencer JP. Aminoglycosides: a practical review. Am Fam Physician. 1998 Nov;58(8):1811–20.

Randjelovic P, Veljkovic S, Stojiljkovic N, Sokolovic D, Ilic I. Gentamicin nephrotoxicity in animals: Current knowledge and future perspectives. EXCLI J [Internet]. 2017 Mar 24;16:388–99. Available from: https://pubmed.ncbi.nlm.nih.gov/28507482

Jiang M, Karasawa T, Steyger PS. Aminoglycoside-Induced Cochleotoxicity: A Review. Front Cell Neurosci. 2017;11:308.

Grill MF, Maganti RK. Neurotoxic effects associated with antibiotic use: management considerations. Br J Clin Pharmacol [Internet]. 2011 Sep;72(3):381–93. Available from: https://pubmed.ncbi.nlm.nih.gov/21501212

Albarellos G, Montoya L, Ambros L, Kreil V, Hallu R, Rebuelto M. Multiple once-daily dose pharmacokinetics and renal safety of gentamicin in dogs. J Vet Pharmacol Ther [Internet]. 2004 Feb 1;27(1):21–5. Available from: https://doi.org/10.1046/j.0140-7783.2003.00545.x

Ross JDC, Brittain C, Cole M, Dewsnap C, Harding J, Hepburn T, et al. Gentamicin compared with ceftriaxone for the treatment of gonorrhoea (G-ToG): a randomised non-inferiority trial. Lancet [Internet]. 2019 Jun 22;393(10190):2511–20. Available from: https://doi.org/10.1016/S0140-6736(18)32817-4

Soeksmanto A, Simanjuntak P, Subroto MA. Uji Toksisitas Akut Ekstrak Air Tanaman Sarang Semut (Myrmecodia pendans) Terhadap Histologi Organ Hati Mencit. J Natur Indones. 2010;12(2):152–5.

Firdausy AF, Nurlaila, Sasmito E. Acute Toxicity of Non-Hexane Fraction of Ethanolic Extract of AntPlant (Myrmecodia tuberose (Jack) Bl.) Hypocotyls in Rats. Int J Pharm Clin Res. 2016;8(1):6–9.

Manullang DH, Sudira IW, Berata IK, Merdana IM. ETHANOL EXTRACT OF MYRMECODIA PENDANS CAUSED HISTOLOGICAL STRUCTURE DAMAGE OF MICE KIDNEY. Bul Vet Udayana; Vol 10 No 2 Agustus 2018DO - 1024843/bulvet2018.v10.i02.p12 [Internet]. 2018 Aug 27; Available from: https://ojs.unud.ac.id/index.php/buletinvet/article/view/34305

Pandia DMH, Wibriansyah A, Pratiwi W, Priadinata AF, Sari W. Uji Toksisitas Akut Ekstrak Etanol Sarang Semut (Myrmecodia pendens) Kalimantan pada Mencit (Mus musculus) Swiss. Pharmamedika Heal Mag. 2011;3(2):189–93.

Yang Y, Yin B, Lv L, Wang Z, He J, Chen Z, et al. Gastroprotective effect of aucubin against ethanol-induced gastric mucosal injury in mice. Life Sci. 2017 Nov;189:44–51.

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