Differences in AGEs-N-Carboxymethyllysine and Kidney Injury Molecule-1 in non-diabetic subjects, diabetic with and without diabetic nephropathy

Dwi Fajaryani, Muji Rahayu, Banundari Rachmawati

Dwi Fajaryani
Resident of Clinical Pathology Department, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Muji Rahayu
Clinical Pathology Department, Faculty of Medicine Universitas Diponegoro, Semarang, Indonesia

Banundari Rachmawati
Clinical Pathology Department, Faculty of Medicine Universitas Diponegoro, Semarang, Indonesia. Email: banundaridr@yahoo.com
Online First: April 30, 2021 | Cite this Article
Fajaryani, D., Rahayu, M., Rachmawati, B. 2021. Differences in AGEs-N-Carboxymethyllysine and Kidney Injury Molecule-1 in non-diabetic subjects, diabetic with and without diabetic nephropathy. Bali Medical Journal 10(1): 325-330. DOI:10.15562/bmj.v10i1.2175

Background: Diabetic nephropathy (ND) is a complication of diabetes mellitus (DM), characterized by persistent albuminuria. N-carboxymethyl lysine (CML) is the most extensive advanced glycation end products (AGEs), formed from the fructoselysine amadori. Kidney Injury Molecule 1 (KIM-1) is a type 1 transmembrane glycoprotein. The aim of the study is to analyze the differences in AGEs-CML and KIM-1 levels in the non-DM subject, DM without and with DN.

Methods: A cross-sectional analytic observational study was conducted on 25 non-DM subjects (K1), 25 DM without DN (K2), and 25 DM with  DN (K3) in PROLANIS Semarang. AGEs-CML and KIM-1 levels were measured using the ELISA method. Inter-group AGEs-CML levels were analyzed using the One way ANOVA test, followed by post hoc Games-Howell. The levels KIM-1 between groups were analyzed using the Kruskal-Wallis test levels, followed by Mann Whitney post hoc test and p<0.05, were considered significant.

Results: There were differences in AGEs-CML levels between K1 (739.89±227.37 ng/ml) and K3 (911.79±107.44) (p = 0.005), between K2 (798.82±153.03) and K3 (911.79 ± 107.44) (p = 0.012) and there was no difference in K1 and K2 (p =0.535). There were differences in KIM-1 level between K1 [9.82 (5.99 – 14.83) pg/ml] and K2 [15.31 (10.12 – 30.21) (p <0.001)], between K1 [9.82 (5.99 – 14.83)] and K3 [15.11 (8.27 – 25.63) (p <0.001)] and there was no difference between K2 and K3 (p=0.720)

Conclusion: The highest AGEs-CML levels were significantly found in the K3 group, followed by K2 and the lowest in K3. KIM-1 levels were significantly found in the K2 group, followed by K3 and the lowest in K1.


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