Anti-diabetic properties of Stevia rebaudiana Bertoni as sugar substitute: a mini-review

Yudi Kristanto , Angeline Rosa Hartono

Yudi Kristanto
Faculty of Medicine Universitas Hang Tuah, Surabaya, Indonesia. Email:

Angeline Rosa Hartono
Faculty of Medicine Universitas Hang Tuah, Surabaya, Indonesia
Online First: April 28, 2021 | Cite this Article
Kristanto, Y., Hartono, A. 2021. Anti-diabetic properties of Stevia rebaudiana Bertoni as sugar substitute: a mini-review. Bali Medical Journal 10(1): 189-193. DOI:10.15562/bmj.v10i1.2259

The incidence of obesity, type II diabetes mellitus, and metabolic syndrome in children and adults show an alarming increase worldwide. This increase was supported by one factor: the increasing availability of high-calorie foods and beverages, thus increasing the need for sweeteners. Researchers are driven to find a sweetener that can replace sugar. One of the natural sweeteners that can be used is the Stevia plant. Stevia contains stevioside, which is a natural sweetener with 300 times sweetness compared to sucrose. Besides, stevioside can reduce blood sugar levels and show benefits for people with diabetes.


Anton SD, Martin CK, Han H, Coulon S, Cefalu WT, Geiselman P, et al. Effects of Stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels. Appetite. 2010/03/18. 2010;55(1):37–43. Available from:

Ludwig DS, Peterson KE, Gortmaker SL. Relation between consumption of sugar-sweetened drinks and childhood obesity: a prospective, observational analysis. Lancet. 2001;357(9255):505–8. Available from:

Kobus-Moryson M, Gramza-Michałowska A. Directions on the use of stevia leaves (Stevia Rebauidana) as an additive in food products. Acta Sci Pol Technol Aliment. 2015;14(1):5–13. Available from:

Puri M, Sharma D, Barrow CJ, Tiwary AK. Optimisation of novel method for the extraction of steviosides from Stevia rebaudiana leaves. Food Chem. 2012;132(3):1113–20. Available from:

Brusick DJ. A critical review of the genetic toxicity of steviol and steviol glycosides. Food Chem Toxicol. 2008;46(7):S83–91. Available from:

Kovylyaeva GI, Bakaleinik GA, Strobykina IY, Gubskaya VI, Sharipova RR, Al'fonsov VA, et al. Glycosides from Stevia rebaudiana. Chem Nat Compd. 2007;43(1):81–5. Available from:

Brahmachari G, Mandal LC, Roy R, Mondal S, Brahmachari AK. Stevioside and Related Compounds - Molecules of Pharmaceutical Promise: A Critical Overview. Arch Pharm (Weinheim). 2010;344(1):5–19. Available from:

Geuns JMC. Stevioside. Phytochemistry. 2003;64(5):913–21. Available from:

Douglas Kinghorn A, Chin Y-W, Pan L, Jia Z. Natural Products as Sweeteners and Sweetness Modifiers [Internet]. Comprehensive Natural Products II. Elsevier; 2010. p. 269–315. Available from:

Ahmed B, Hossain M, Islam R, Kumar Saha A, Mandal A. A review on natural sweetener plant–Stevia having medicinal and commercial importance. Agron Glas Glas Hrvat Agron društva. 2011;73(1–2):75–91.

Mosettig E, Beglinger U, Dolder F, Lichti H, Quitt P, Waters JA. The Absolute Configuration of Steviol and Isosteviol. J Am Chem Soc. 1963;85(15):2305–9. Available from:

Savita SM, Sheela K, Sunanda S, Shankar AG, Ramakrishna P. Stevia rebaudiana – A Functional Component for Food Industry. J Hum Ecol. 2004;15(4):261–4. Available from:

Rafiq M, Dahot MU, Mangrio SM, Naqvi HA, Qarshi IA. In vitro clonal propagation and biochemical analysis of field established Stevia rebaudiana Bertoni. Pak J Bot. 2007;39(7):2467–74.

Abou-Arab EA, Abou-Arab A, Abu-Salem F. PHYSICO-CHEMICAL ASSESSMENT OF NATURAL SWEETENERS STEVIOSIDES PRODUCED FROM STEVIA Rebudiana bertoni PLANT. J Food Dairy Sci. 2009;34(12):11037–57. Available from:

Kim I-S, Yang M, Lee O-H, Kang S-N. The antioxidant activity and the bioactive compound content of Stevia rebaudiana water extracts. LWT - Food Sci Technol. 2011;44(5):1328–32. Available from:

Gardana C, Scaglianti M, Simonetti P. Evaluation of steviol and its glycosides in Stevia rebaudiana leaves and commercial sweetener by ultra-high-performance liquid chromatography-mass spectrometry. J Chromatogr A. 2010;1217(9):1463–70. Available from:

Atteh JO, Onagbesan OM, Tona K, Decuypere E, Geuns JMC, Buyse J. Evaluation of supplementary Stevia (Stevia rebaudiana,bertoni) leaves and stevioside in broiler diets: effects on feed intake, nutrient metabolism, blood parameters and growth performance. J Anim Physiol Anim Nutr (Berl). 2008;92(6):640–9. Available from:

Brandle JE, Telmer PG. Steviol glycoside biosynthesis. Phytochemistry. 2007;68(14):1855–63. Available from:

Geuns JMC, Buyse J, Vankeirsbilck A, Temme EHM. Metabolism of stevioside by healthy subjects. Exp Biol Med. 2007;232(1):164–73.

Koyama E, Kitazawa K, Ohori Y, Izawa O, Kakegawa K, Fujino A, et al. In vitro metabolism of the glycosidic sweeteners, stevia mixture and enzymatically modified Stevia in human intestinal microflora. Food Chem Toxicol. 2003;41(3):359–74. Available from:

Chatsudthipong V, Muanprasat C. Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacol Ther. 2009;121(1):41–54. Available from:

Geuns JMC, Augustijns P, Mols R, Buyse JG, Driessen B. Metabolism of stevioside in pigs and intestinal absorption characteristics of stevioside, rebaudioside A and steviol. Food Chem Toxicol. 2003;41(11):1599–607. Available from:

Wang LZ, Goh BC, Fan L, Lee HS. Sensitive high-performance liquid chromatography/mass spectrometry method for determination of steviol in rat plasma. Rapid Commun Mass Spectrom. 2003;18(1):83–6. Available from:

Megeji NW, Kumar JK, Singh V, Kaul VK, Ahuja PS. Introducing Stevia rebaudiana, a natural zero-calorie sweetener. Curr Sci. 2005;801–4.

Curi AM, Bazotte RB, Botion LM, Godoy JL, Bracht A. Effect of Stevia rebaudiana on glucose tolerance in normal condition. Brazil Media Litbang Kesehat. 2011;21.

Jeppesen PB, Gregersen S, Poulsen CR, Hermansen K. Stevioside acts directly on pancreatic β cells to secrete insulin: Actions independent of cyclic adenosine monophosphate and adenosine triphosphate—sensitivie K+-channel activity. Metabolism. 2000;49(2):208–14. Available from:

Jyoti J, Kaur M, Mishra V, Mittal A. Sweet future of Stevia: a magical sweetener. Asian J Pharm Clin Res. 2018;11(2):36–42.

Gregersen S, Jeppesen PB, Holst JJ, Hermansen K. Antihyperglycemic effects of stevioside in type 2 diabetic subjects. Metabolism. 2004;53(1):73–6. Available from:

Shivanna N, Naika M, Khanum F, Kaul VK. Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana. J Diabetes Complications. 2013;27(2):103–13. Available from:

Wang Z, Xue L, Guo C, Han B, Pan C, Zhao S, et al. Stevioside ameliorates high-fat diet-induced insulin resistance and adipose tissue inflammation by downregulating the NF-κB pathway. Biochem Biophys Res Commun. 2012;417(4):1280–5. Available from:

Unger RH. Role of glucagon in the pathogenesis of diabetes: The status of the controversy. Metabolism. 1978;27(11):1691–709. Available from:

Erkucuk A, Akgun IH, Yesil-Celiktas O. Supercritical CO2 extraction of glycosides from Stevia rebaudiana leaves: Identification and optimization. J Supercrit Fluids. 2009;51(1):29–35. Available from:

Abudula R, Jeppesen PB, Rolfsen SED, Xiao J, Hermansen K. Rebaudioside A potently stimulates insulin secretion from isolated mouse islets: Studies on the dose-, glucose-, and calcium-dependency. Metabolism. 2004;53(10):1378–81. Available from:

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