Amino acid methionine, lysine and vitamin choline: A potential feed supplement to reduce mortality, improve immunity, and health of Bali pigs in the growth phase

Ida Bagus Komang Ardana; Ketut Sumadi; Ketut Budiasa; Anak Agung Gde Oka Dhamayudha; Hamong Suhasono; Nico Fajar Gunawan

doi:10.15562/bmj.v10i2.2363

Amino acid methionine, lysine and vitamin choline: A potential feed supplement to reduce mortality, improve immunity, and health of Bali pigs in the growth phase

Ida Bagus Komang Ardana ,

Ketut Sumadi ,

Ketut Budiasa ,

Anak Agung Gde Oka Dhamayudha ,

Hamong Suhasono ,

Nico Fajar Gunawan ,

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DOI: https://doi.org/10.15562/bmj.v10i2.2363 |
Published: 2021-08-21

Abstract

Introduction: Balinese piglets, separated from the mother and given traditional feeding, can cause prolonged growth. So, it is necessary to provide feed with a complete formula to support the growth process. Previous studies reported that Balinese piglets fed with a mixture of yellow corn, pollard, Aminovite, table salt, and minerals with several formulations and a metabolized energy (ME)/crude protein (CP) ratio 2800 kcal/kg/12% significantly increased body weight. However, his health status has not been reported, both the hematological profile and antibody titer. The purpose of this study was to determine the effect of the amino acid methionine, lysine, and choline (Aminovite) on health, hematological profile, and antibody titer as immunomodulatory effects.

Methods: This study used 72 Balinese piglets after being separated from the mother, divided into four groups of 18 piglets each. The first group (P0) was given basic feed mixtures of corn and pollard as a control. The second group (P1) was fed P0 + 0.5% Aminovite. The third group (P2) was fed P0 + 1.00% Aminovite. The fourth group (P3) was fed P0 + 1.50% Aminovite. Bali piglets are vaccinated with the Hog Cholera vaccine. Furthermore, on the 12th day of the week after treatment (aged 80-90 days), a hematological profile examination will be carried out, and the antibody titer of Hog Cholera will be examined. The data were analyzed using the One Way Anova test and Duncan's Multiple Range Test. The p-value <0.05 is significant.

Result: There was no mortality of piglets in all groups, but the control group had a high morbidity rate (22.2%) than the other groups. Aminovite mixed in the feed significantly increased WBC, total RBC, Hb levels, and PCV values compared to control (p<0.05). Aminovite can also increase neutrophils and monocytes and significantly reduce lymphocytes compared to control (p<0.05).

Conclusion: The addition of Aminovite as a feed supplement in Balinese piglet feed improved the health of Bali piglets seen from the blood profile and increased Antibody titers resulting from Hog Cholera vaccination.

References

Mpetile Z, Young JM, Gabler NK, Dekkers JCM, Tuggle CK. Assessing peripheral blood cell profile of Yorkshire pigs divergently selected for residual feed intake. J Anim Sci. 2015;93(3):892. Available from: http://dx.doi.org/10.2527/jas.2014-8132
Ardana B, Putra H. Manajemen Reproduksi, Produksi, dan Penyakit Ternak Babi. Denpasar: Udayana University Press. Bali; 2008.
Gloaguen M, Le Floc'h N, Primot Y, Corrent E, van Milgen J. Response of piglets to the standardized ileal digestible isoleucine, histidine and leucine supply in cereal–soybean meal-based diets. Animal. 2013;7(6):901–8. Available from: http://dx.doi.org/10.1017/s1751731112002339
Astawa IPARI, Wibawa A, Puger AW. Pengaruh penambahan campuran asam amino esensial dan kolin (aminovit) dalam pakan tradisional terhadap penampilan babi bali jantan. Maj Ilm Peternak. 2018;21(1):32–6.
Jain NC. Schalm's veterinary hematology. Lea & Febiger; 1986.
Wang D, Chen G, Song L, Chai M, Wang Y, Shui S, et al. Effects of Dietary Protein Levels on Bamei Pig Intestinal Colony Compositional Traits [Internet]. Authorea, Inc.; 2020. Available from: http://dx.doi.org/10.22541/au.159551270.00410123
Stegeman JA, Bouma A, Elbers AR, Verheijden JH. The leukocyte count is a valuable parameter for detecting classical swine fever. Tijdschr Diergeneeskd. 2000;125(17):511–8.
National Research Council (US). Protein and Amino Acids. In: Recommended Dietary Allowances. 10th ed. Washington (DC): National Academies Press (US); 1989. Available from: https://www.ncbi.nlm.nih.gov/books/NBK234922/
Fuller MF, McWilliam R, Wang TC, Giles LR. The optimum dietary amino acid pattern for growing pigs: 2. Requirements for maintenance and for tissue protein accretion. Br J Nutr. 1989;62(2):255–67.
Gloaguen M, Le Floc’h N, Corrent E, Primot Y, van Milgen J. The use of free amino acids allows formulating very low crude protein diets for piglets1. J Anim Sci. 2014;92(2):637–44. Available from: http://dx.doi.org/10.2527/jas.2013-6514
Giles TA, Belkhiri A, Barrow PA, Foster N. Molecular approaches to the diagnosis and monitoring of production diseases in pigs. Res Vet Sci. 2017/05/17. 2017;114:266–72. Available from: https://pubmed.ncbi.nlm.nih.gov/28535467