ORIGINAL ARTICLE

Analysis of sea bidara leaf (Ziziphus mauritiana) ethanol extract biorespon on hydrophobicity and phospholipase of Streptococcus pyogenes cell surface in tonsillitis isolate

Benny Kurnia , Setiani Lily, R. Ega Suharno

Benny Kurnia
Department of Otorhinolaryngology - Head and Neck Surgery, Universitas Syiah Kuala, Banda Aceh, Indonesia. Email: kpstht_fku@yahoo.com

Setiani Lily
Department of Otorhinolaryngology - Head and Neck Surgery, Universitas Syiah Kuala, Banda Aceh, Indonesia

R. Ega Suharno
Department of Otorhinolaryngology - Head and Neck Surgery, Universitas Syiah Kuala, Banda Aceh, Indonesia
Online First: July 08, 2021 | Cite this Article
Kurnia, B., Lily, S., Suharno, R. 2021. Analysis of sea bidara leaf (Ziziphus mauritiana) ethanol extract biorespon on hydrophobicity and phospholipase of Streptococcus pyogenes cell surface in tonsillitis isolate. Bali Medical Journal 10(2): 572-578. DOI:10.15562/bmj.v10i2.2419


Background: Tonsillitis is an inflammation of the palatine tonsil that is often found in otorhinolaryngology and is one of the world's socio-economic health problems. Streptococcus pyogenes is the most common bacterial cause of tonsillitis, with hydrophobicity and phospholipase activity on the cell surface that has a major effect on tonsillitis pathogenicity. Ethanol extract of the leaves of sea bidara (Ziziphus mauritiana) has an antibacterial impact containing neophytadiene, hexadecanoic acid, linolenic acid, octadecanoic acid, squalene, and vitamin E.

Objective: To determine the ability of sea bidara leaf (Ziziphus mauritiana) ethanol extract inhibiting the hydrophobicity and phospholipase activity of the Streptococcus pyogenes cell surface in tonsillitis isolate. Methods: Laboratory experimental design with a post-test-only control group.

Result: Ethanol extract of Ziziphus mauritiana leaf could inhibit the surface hydrophobicity activity of Streptococcus pyogenes cell with different quantity and quality at each concentration and incubation time. Analysis of independent T test (p>0.05;0.965) and (p>0.05;0.683) proved that there was no significant difference in incubation time inhibiting hydrophobicity and phospholipase activity of Streptococcus pyogenes cell surface. Kruskal-Wallis analysis (p>0.05;0.689) and (p>0.05;0.162) proved that there was no significant difference in inhibiting the hydrophobicity and phospholipase activity of Streptococcus pyogenes cell surface.

Conclusion: Ethanol extract of Ziziphus mauritiana leaf can inhibit the hydrophobicity and phospholipase activity of Streptococcus pyogenes cell surface in isolate tonsillitis.

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