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Growth factors of hypoxia Freeze-Dried Adipose Stem Cell- Conditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM): a comparative study

  • Winawati Eka Putri ,
  • Anang Endaryanto ,
  • Damayanti Tinduh ,
  • Fedik Rantam ,
  • Medhi Denisa Alinda ,
  • Cita Rosita Sigit Prakoeswa ,

Abstract

Background: Adipose Stem Cells (ASC) are multipotent stem cells which mechanism of action is mainly secretion of various paracrine molecules known as secretome or conditioned medium (CM). Adipose Stem Cell-Conditioned Medium (ASC-CM) collects several soluble factors, such as cytokines, chemokines, and Growth Factors (GF) secreted by almost all types of living cells in the extracellular space. The freeze-dried (FD) form of ASC-CM can reduce the instability of the fresh (FR) form of ASC-CM. This study aims to compare the GF levels between FD ASC-CM and FR ASC-CM as a comparative study.

Methods: ASC-CM was prepared from human adipose tissue. After we got the FR ASC-CM, some of them were changed into an FD form. We measured the several GF levels using ELISA methods in ng/L. Data were presented in mean and standard deviation as well as analyzed using SPSS version 25 for Windows.

Results: The average levels of EGF in both FD ASC-CM and FR ASC-CM were 256.80±9.70 ng/L and 290.20±53.20 ng/L, followed by TGF-? (564.20±12.70 ng/L and 633.10±11.40 ng/L), PDGF (2.90±0.19 ng/L and 2.90±0.20 ng/L), and VEGF (711.10±21.70 ng/L and 788.70±204.80 ng/L) respectively. There was a statistically significant difference in TGF-? levels between FD ASC-CM and FR ASC-CM (p=0.002). However, there was no a significant difference in EGF, PDGF, and VEGF levels between FD ASC-CM and FR ASC-CM (p>0.05)

Conclusion: There was a statistically significant difference between FD and FR ASC-CM only in TGF-? levels.

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How to Cite

Putri, W. E., Endaryanto, A., Tinduh, D., Rantam, F., Alinda, M. D., & Prakoeswa, C. R. S. (2021). Growth factors of hypoxia Freeze-Dried Adipose Stem Cell- Conditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM): a comparative study. Bali Medical Journal, 10(2), 683–687. https://doi.org/10.15562/bmj.v10i2.2588

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Winawati Eka Putri
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Anang Endaryanto
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Damayanti Tinduh
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Fedik Rantam
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Medhi Denisa Alinda
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Cita Rosita Sigit Prakoeswa
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