Skip to main content Skip to main navigation menu Skip to site footer

Growth factors of hypoxia Freeze-Dried Adipose Stem Cell- Conditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM): a comparative study

  • Winawati Eka Putri ,
  • Anang Endaryanto ,
  • Damayanti Tinduh ,
  • Fedik Rantam ,
  • Medhi Denisa Alinda ,
  • Cita Rosita Sigit Prakoeswa ,


Abstract

Background: Adipose Stem Cells (ASC) are multipotent stem cells which mechanism of action is mainly secretion of various paracrine molecules known as secretome or conditioned medium (CM). Adipose Stem Cell-Conditioned Medium (ASC-CM) collects several soluble factors, such as cytokines, chemokines, and Growth Factors (GF) secreted by almost all types of living cells in the extracellular space. The freeze-dried (FD) form of ASC-CM can reduce the instability of the fresh (FR) form of ASC-CM. This study aims to compare the GF levels between FD ASC-CM and FR ASC-CM as a comparative study.

Methods: ASC-CM was prepared from human adipose tissue. After we got the FR ASC-CM, some of them were changed into an FD form. We measured the several GF levels using ELISA methods in ng/L. Data were presented in mean and standard deviation as well as analyzed using SPSS version 25 for Windows.

Results: The average levels of EGF in both FD ASC-CM and FR ASC-CM were 256.80±9.70 ng/L and 290.20±53.20 ng/L, followed by TGF-? (564.20±12.70 ng/L and 633.10±11.40 ng/L), PDGF (2.90±0.19 ng/L and 2.90±0.20 ng/L), and VEGF (711.10±21.70 ng/L and 788.70±204.80 ng/L) respectively. There was a statistically significant difference in TGF-? levels between FD ASC-CM and FR ASC-CM (p=0.002). However, there was no a significant difference in EGF, PDGF, and VEGF levels between FD ASC-CM and FR ASC-CM (p>0.05)

Conclusion: There was a statistically significant difference between FD and FR ASC-CM only in TGF-? levels.

Keywords: ASC-CM Growth Factors Freeze-Dried Fresh.

References

  1. Park BS, Kim WS, Choi JS, Kim HK, Won JH, Ohkubo F, Fukuoka H. Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion. Biomed Res. 2010;31(1):27-34. doi: 10.2220/biomedres.31.27. PMID: 20203417
  2. Hasegawa, T., & Ikeda, S. Mesenchymal Stem Cells for the Treatment of Skin Diseases. AIMS Cell and Tissue Engineering. 2017;1(2), 104.
  3. Bruno S., Collino F., Deregibus M. C., Grange C., Tetta C., Camussi G. Microvesicles derived from human bone marrow mesenchymal stem cells inhibit tumor growth. Stem Cells Dev. 2013;22, 758–771. 10.1089/scd.2012.0304
  4. Collawn, Sherry S & Patel, Shyam. Adipose-Derived Stem Cells, their Secretome, and Wound Healing. Journal of Cell Science & Therapy. 2014;5:3 DOI: 10.4172/2157-7013.1000165
  5. Tran C, Damaser MS. Stem cells as drug delivery methods: application of stem cell secretome for regeneration. Adv Drug Deliv. 2015;82-83:1-11. doi: 10.1016/j.addr.2014.10.007. Epub 2014 Oct 15. PMID: 25451858; PMCID: PMC4398586.
  6. Phelps, J., Sanati-nezhad, A., Ungrin, M., Duncan, N. A., & Sen, A. Review Article Bioprocessing of Mesenchymal Stem Cells and Their Derivatives : Toward Cell-Free Therapeutics. 2018;(iii).
  7. Lombardi, F., Palumbo, P., Augello, F. R., Cifone, M. G., Cinque, B., & Giuliani, M. Secretome of adipose tissue-derived stem cells (ASCs) as a novel trend in chronic non-healing wounds: An overview of experimental in vitro and in vivo penelitianes and methodological variables. International Journal of Molecular Sciences. 2019;20(15). https://doi.org/10.3390/ijms20153721
  8. Noverina, R., Widowati, W., Ayuningtyas, W., Kurniawan, D., Afifah, E., Laksmitawati, D. R., … Wirakusumah, F. F. Growth Factors Profile in Conditioned Medium human Adipose Tissue-derived Mesenchymal Stem Cells (CM-hATMSCs). Clinical Nutrition Experimental.2019. doi:10.1016/j.yclnex.2019.01.002
  9. Lee, S. E., Kwon, T. R., Kim, J. H., Lee, B. C., Oh, C. T., Im, M., Hwang, Y. K., Paik, S. H., Han, S., Kim, J. Y., & Kim, B. J. Anti?Photoaging and anti?oxidative activities of natural killer cell conditioned medium following UV?B irradiation of human dermal fibroblasts and a reconstructed skin model. International Journal of Molecular Medicine. 2019;44(5), 1641– 1652. https://doi.org/10.3892/ijmm.2019.4320
  10. Seo, Y., Shin, T. H., & Kim, H. S. Current strategies to enhance adipose stem cell function: An update. International Journal of Molecular Sciences. 2019;20(15), 3837. https://doi.org/10.3390/ijms20153827
  11. Bertozzi, N., Simonacci, F., Grieco, M. P., Grignaffini, E., & Raposio, E. Adipose-derived stem cells as a novel anti-aging therapy in cosmetic surgery: a concise review. Euromediterranean Biomedical Journal. 2018;13(10), 46–56.
  12. Bari E, Perteghella S, Silvestre DD, Sorlini M, Catenacci L, Sorrenti M, Marrubini G, Rossi
  14. R, Tripodo G, Mauri P, Marazzi <, Torre ML. Pilot production of mesenchymal stem/stromal freeze-dried secretome for Cell-free regenerative nanomedicine: a validated GMP-compliant process. Cells. 2018. Vol. 7
  15. Nireesha, G., Divya, L., Sowmya, C., Venkateshan, N., Babu, M., & Lavakumar, V. Lyophilization/Freeze Drying - An Review. International Journal Of Novel Trends In Pharmaceutical Sciences. 2018;3(4): 87-98
  16. Peng, Y., Xuan, M., Zou, J., Liu, H., Zhuo, Z., Wan, Y., & Cheng, B. Freeze-Dried Rat Bone Marrow Mesenchymal Stem Cell Paracrine Factors: A Simplified Novel Material for Skin Wound Therapy. Tissue Engineering Part A. 2015;21(5-6), 1036–1046. doi:10.1089/ten.tea.2014.0102
  17. Ma, S., Xie, N., Li, W., Yuan, B., Shi, Y., & Wang, Y. Immunobiology of mesenchymal stem cells. Cell Death & Differentiation. 2013;21(2), 216–225. doi:10.1038/cdd.2013.158
  18. Zhang S, Duan E. 2018. Fighting against Skin Aging: The Way from Bench to Bedside. Cell Transplant. 27(5):729-738. doi: 10.1177/0963689717725755. Epub 2018 Apr 25. PMID: 29692196; PMCID: PMC6047276
  19. Baldari, S., Di Rocco, G., Piccoli, M., Pozzobon, M., Muraca, M., & Toietta, G. Challenges and strategies for improving the regenerative effects of mesenchymal stromal cell-based therapies. International Journal of Molecular Sciences. 2017;18(10), 2087.
  20. Jayaraman, P., Nathan, P., Vasanthan, P., & Musa, S. Stem cells conditioned medium : a new approach to skin wound healing management. 2013;37, 1122–1128. https://doi.org/10.1002/cbin.10138
  21. Zhou, B.-R., Xu, Y., Guo, S.-L., Xu, Y., Wang, Y., Zhu, F., … Luo, D. The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing. BioMed Research International. 2013;1–9. doi:10.1155/2013/519126
  22. Dubey NK, Mishra VK, Dubey R, Deng Y-H, Tsai F-C, Deng W-P. ‘Revisiting the Advances in Isolation, Characterization and Secretome of Adipose-Derived Stromal/Stem Cells’ International Journal of Molecular Sciences. 2018;19(8):2200. https://doi.org/10.3390/ijms19082200.
  23. Bogatcheva, N. V, & Coleman, M. E. Conditioned Medium of Mesenchymal Stromal Cells : A New Class of Therapeutics. 2019;84(11), 1375–1389.
  24. Gwam, C., Mohammed, N., & Ma, X. Stem cell secretome , regeneration , and clinical translation : a narrative review. 2021;9(6), 1–11. https://doi.org/10.21037/atm-20-5030
  25. Ellab Validation Solutions. The Freeze Drying Theory and Process - Things to Consider. Ellab White Paper. 2018;1–16. Retrieved from https://www.ellab.com/Files/Files/White- Papers/The-Freeze-Drying-Theory-and-Process_ellab-whitepaper.pdf
  26. He, Y., Xia, J., Chen, H., Wang, L., Deng, C., & Lu, F. Human adipose liquid extract induces angiogenesis and adipogenesis: a novel cell-free therapeutic agent. Stem Cell Research & Therapy.2019;10(1). doi:10.1186/s13287-019-1356-0
  27. Joseph A, Baiju I, Bhat IA, Pandey S, Bharti M, Verma M, Singh AP, Ansari MM, Chandra
  29. V, Saikumar G, Amarpal, Sharma GT. Mesenchymal stem cell-conditioned medium: a novel alternative of stem cell therapy for quality wound healing. J cell physiol. 2020
  30. Sun B, Guo S, Wang B, Liu X, Zhang Y, X u Y. Concentrated hypoxia-preconditioned adipose mesenchymal stem cell-conditioned medium improves wounds healing in full- thickness skin defect model.International scholarly research notices. Vol. 2014 Zhou BR, Xu YI, Guo SL2, Xu Y2, Wang Y2, et al. 2013. The effect of conditioned media of adipose- derived stem cells on wound healing after ablative fractional carbon dioxide laser resurfacimg. Biomed Res Int.2014;519126
  31. Huber, S. C., Junior, J. L. R. C., Silva, L. Q., Montalvão, S. A. L., & Annichino-Bizzacchi, J.
  32. M. Freeze-dried versus fresh platelet-rich plasma in acute wound healing of an animal model. Regenerative Medicine. 2019 doi:10.2217/rme-2018-0119
  33. Hsiao ST-F, Asgari A, Lokmic Z, Sinclair R, Dusting GJ, Lim SY, Dilley RJ. Comparative analysis of paracrine factor expression in human adult mesenchymal stem cells derived from bone marrow, adipose,and dermal tissue. Stem Cells and Development. 2012;21(12): 2189– 2203. https://doi.org/10.1089/scd.2011.0674 [PubMed] [PMC]

How to Cite

Putri, W. E., Endaryanto, A., Tinduh, D., Rantam, F., Alinda, M. D., & Prakoeswa, C. R. S. (2021). Growth factors of hypoxia Freeze-Dried Adipose Stem Cell- Conditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM): a comparative study. Bali Medical Journal, 10(2), 683–687. https://doi.org/10.15562/bmj.v10i2.2588
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
0

Total
16

Share

Search Panel

Winawati Eka Putri
Google Scholar
Pubmed
BMJ Journal

Anang Endaryanto
Google Scholar
Pubmed
BMJ Journal

Damayanti Tinduh
Google Scholar
Pubmed
BMJ Journal

Fedik Rantam
Google Scholar
Pubmed
BMJ Journal

Medhi Denisa Alinda
Google Scholar
Pubmed
BMJ Journal

Cita Rosita Sigit Prakoeswa
Google Scholar
Pubmed
BMJ Journal