Background: Increasing incidence of HIV infection in pregnancy will increase the risk of mother to child transmission of HIV. The objectives of prevention of mother to child transmission (PMTCT) is to prevent the mother to child HIV transmission and HIV infection epidemic. Our study aimed to provide data on characteristics of pregnant women with HIV infection following PMTCT Program services in Sanglah General Hospital. Methods: A descriptive study was held by reviewing medical records of pregnant women with HIV infection following PMTCT program in Nigraha Clinic Sanglah General Hospital during 2005-2014 period. Results: In 10 yearsâ€™ period (2005-2014), out of 11,019 pregnant women 273 cases (2.48%) were pregnancy with HIV. Most cases were found in 20-29 years old group (68.86%), had finished high school education (57.50%), working as full-time housewives (67.39%), came from Denpasar (39.56%). The most risk factors were heterosexual husband (46.88%). Most cases were para 1 (42.85%), referred from Sanglah General Hospital VCT Clinic (40.65%), first visit PMTCT clinic on more than 28 weeksâ€™ gestational age (48.71%), found at HIV stage I (70,32%). 43.22% had been taking Antiretroviral (ARV) for more than 6 months. The mode of delivery was cesarean section (CS) (60.16%), at 37-42 weeks of pregnancy (73.58%). Most infants were born with birth weight more than 2500 gram (72.65%) and with non-reactive test result (50%). Conclusions: Prevention of Mother to Child Transmission of HIV (PMTCT) in Sanglah General Hospital is effective in preventing mother to child transmission of HIV.