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Phytochemical screening and toxicological evaluation using Brine Shrimp Lethality Tes (BSLT) of ethanolic extract of Morinda citrifolia L.

  • Devyana Dyah Wulandari ,
  • Reine Risa Risthanti ,
  • Elviana Adhaini Puspita Sari ,
  • Hikmatun Anisa ,
  • Sisca Filia ,

Abstract

Introduction: Noni, which has the scientific name Morinda citrifolia L., is a plant often found in Indonesia and has many properties used for generations. However, this noni fruit has potential toxicity that needs to be studied further. This study aimed to determine the phytochemical compounds and determine the toxicity of the ethanol extract of noni fruit using the Brine Shrimp Lethality Test (BSLT) method.

Methods: The research was conducted at the Chemistry Laboratory, Universitas Nahdlatul Ulama Surabaya. This research includes experimental research. Artemia salina L. larvae aged 48 hours were put into a test tube containing a solution of noni fruit extract with concentrations of 10, 50, 100, 250, 500, 750, and 1000 ppm, then observed after 24 hours.

Results: In the phytochemical screening results, noni fruit ethanol extract contains tannins, saponins, flavonoids, and terpenoids. The test results obtained an LC50 value of 17.78 ppm, which indicates that the ethanol extract of the noni fruit is toxic.

Conclusion: The ethanol extract of the noni fruit contains tannins, saponins, flavonoids, and terpenoids and has toxicity to Artemia salina L. larvae. It can be concluded that the ethanolic extract of noni fruit has the potential as an anticancer.

References

  1. Piaru SP, Mahmud R, Abdul Majid AMS, Mahmoud Nassar ZD. Antioxidant and antiangiogenic activities of the essential oils of Myristica fragrans and Morinda citrifolia. Asian Pac J Trop Med. 2012;5(4):294–8. Available from: http://dx.doi.org/10.1016/S1995-7645(12)60042-X
  2. Krishnaiah D, Bono A, Sarbatly R, Anisuzzaman SM. Antioxidant activity and total phenolic content of an isolated Morinda citrifolia L. methanolic extract from Poly-ethersulphone (PES) membrane separator. J King Saud Univ - Eng Sci. 2015;27(1):63–7. Available from: http://dx.doi.org/10.1016/j.jksues.2013.01.002
  3. Kaur H, Gurjar N, Gill R. The noni fruit (Morinda citrifolia L.): A systematic review on anticancer potential and other health beneficial pharmacological activ ities. 2018;86–93.
  4. Almeida-Souza F, De Oliveira AER, Abreu-Silva AL, Da Silva Calabrese K. In vitro activity of Morinda citrifolia Linn. fruit juice against the axenic amastigote form of Leishmania amazonensis and its hydrogen peroxide induction capacity in BALB/c peritoneal macrophages. BMC Res Notes. 2018;11(1):1–7. Available from: https://doi.org/10.1186/s13104-018-3555-7
  5. Nirawati C. Uji Daya Hambat Ekstrak Daun dan Buah Mengkudu (Morinda citrifolia L.) Terhadap Pertumbuhan Bakteri Escherichia coli Sebagai Penunjang Praktikum Mata Kuliah Mikrobiologi. Universitas Islam Negeri AR. RANIRY Darussalam; 2016.
  6. Ariyanto W, Sadimin S, Sariyem S. Daya Hambat Ekstrak Biji Mengkudu Terhadap Pertumbuhan Bakteri Streptococcus Mutans. J Kesehat Gigi. 2016;3(1):34–40.
  7. Fikri A, Mugiyanto E. Uji Toksisitas Ekstrak Etanol Daun, Daging Buah Dan Biji Mengkudu (Morinda citrifolia Linn.) Dengan Metode BSLT (Brine Shrimp Lethality Test). Jurnal Farmasi dan Kesehatan. Uji Toksisitas Ekstrak Etanol Daun, Daging Buah Dan Biji Mengkudu (Morinda citrifolia Linn) Dengan Metod BSLT (Brine Shrimp Lethality Test) J Farm dan Kesehatan. 2018;1(2):1–8.
  8. Antonius P, Eko M. Uji Toksisitas Daun Ketepeng (Cassia Alata L.), Kulit Buah Pisang Ambon (Musa Paradisiaca L. Var Sapientum) dan Kulit Rimpang Kencur (Kaempferia Galanga Linn.) dengan Metode Brine Shrimp Lethality Test (BSLT). In: Proceeding of The URECOL. 2018. p. 189–94.
  9. Maryati A, Khairudin M. Aktivitas Sitotoksik Ekstrak Heksan, Ekstrak Etil Asetat, Ekstrak Etanol Buah Mengkudu (Morinda citrifolia L ) Terhadap Sel T47D. J Farm Indones. 2020;17(1):1–10.
  10. Juhaeriah E. Uji Toksisitas Akut Ekstrak Etanol Daun Paku Pedang (Nepholepis Falcata) Terhadap Larva Artemia salina Leach Dengan Metode BSLT. Universitas Islam Negeri Syarif Hidayatullah.; 2013.
  11. Sangi MS, Momuat LI, Kumaunang M. Uji Toksisitas Dan Skrining Fitokimia Tepung Gabah Pelepah Aren (Arenga Pinnata). J Ilm Sains. 2012;12(2):127.
  12. Khotimah K. Skrining Fitokimia dan Identifikasi Metabolit Sekunder Senyawa Karpain Pada Ekstrak Metanol Daun Carica pubescens Lenne dan K. Koch Dengan LC/MS. Uin Maulana Malik Ibrohim Malang. 2016.
  13. Rustina. Uji Aktivitas Antioksidan dan Antibakteri Ekstrak Etil Asetat Labu Kuning (Cucuma moschata Duch.Poir). Universitas Muhammadiyah Yogyakarta; 2016.
  14. Supomo S, Warnida H, Said BM. Uji Aktivitas Antioksidan Ekstrak Etanol Umbi Bawang Rambut (Allium chinense G.Don) Dengan Penagkal Radikal DPPH (1,1- Difenil-2-Pikrilhidrazil). J Ris Kefarmasian Indones. 2019;1(1):30–40.
  15. Dwijayanti E, Alimuddin AH, Wibowo MA. Skrining fitokimia dan uji aktivitas sitotoksik pada kulit batang tampoi (Baccaurea macrocarpa) terhadap Artemia Salina Leach dengan Metode BSLT. J Kim Khatulistiwa. 2014;3(4):6–10.
  16. Woo HD, Kim J. Dietary flavonoid intake and risk of stomach and colorectal cancer. World J Gastroenterol. 2013;19(7):1011–9.
  17. Albuntana A, Yasman, Wardhana W. Toksisitas Ekstrak Empat Jenis Teripang Suku Holothuridae Dari Pulau Penjaliran Timur, Kepulauan Seribu, Jakarta, Menggunakan Brine Shrimp Lethality Test. J Ilmu dan Teknol Kelaut Trop. 2011;3(1):65–725.
  18. Kramy P, Triawanti A. Uji Sitotoksik Ekstrak Etanol Caun Tumbuhan Jinjit (Desmodium Heterocarpon) terhadap Larva Artemia Salina Leach dengan Metode Brine Shrimp Lethality Test (BSLT). J Kedokt Univ Palangka Raya. 2017;2(1):1.

How to Cite

Wulandari, D. D., Risthanti, R. R., Sari, E. A. P., Anisa, H., & Filia, S. (2022). Phytochemical screening and toxicological evaluation using Brine Shrimp Lethality Tes (BSLT) of ethanolic extract of Morinda citrifolia L. Bali Medical Journal, 11(2), 561–565. https://doi.org/10.15562/bmj.v11i2.3119

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Devyana Dyah Wulandari
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Reine Risa Risthanti
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Elviana Adhaini Puspita Sari
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Hikmatun Anisa
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Sisca Filia
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