Objective: Group-B Streptococci (GBS) are Gram-positive cocci that are the most commonÂ cause of early onset neonatal sepsis. The mortality rate of early onset neonatal sepsis has beenÂ reported up to 50%. One of the major risks of early onset neonatal sepsis is GBS colonization in birth
canal of pregnant women that can infect the baby during process of vaginal delivery. AntibioticÂ chemoprophylaxis for pregnant women that is colonized by GBS can reduce the risk of early onsetÂ neonatal sepsis. The detection of GBS colonization needs Todd Hewitt (TH) enrichment medium to
reduce false negative result. Until now, there is no report about either prevalence of colonization orÂ sensitivity pattern of Group B Streptococcus among pregnant women in Denpasar. The aims of thisÂ research were to determine the prevalence of GBS colonization and sensitivity pattern of GBS among
pregnant women with Todd Hewitt enrichment medium.
Method: This research was a descriptiveÂ cross-sectional study. Vaginal swab specimens from 35ï€37 weeks gestation pregnant women wereÂ collected and 32 samples that met the inclusion criteria were cultured on Blood agar (BA) plates,Â Chromagar (CA) plates, and Todd Hewitt (TH) broth. The GBS colonization that grew in cultureÂ medium was followed by antibiotic sensitivity test.
Results: In the present study, we found that theÂ prevalence of GBS colonization in pregnant women detected with culture method using BA and CAÂ without TH broth was 9.4%, whereas the prevalence with culture method using BA and CA enrichedÂ by TH broth was 31.3%. Moreover, GBS showed resistance to penicillin, erythromycin, and cefazolin.Â It is indicated that TH enrichment medium seems to be promising as a screening method for GBSÂ colonization in pregnant women in Bali.
Conclusion: There was an enrichment detection of GBSÂ prevalence colonization in pregnant women detected the swab with culture method using BA and CAÂ enriched by TH compare to BA and CA without TH broth. Moreover, GBS showed resistance toÂ penicillin, erythromycin, and cefazolin. It is indicated that TH enrichment medium seems to be
promising as a screening method for GBS colonization in pregnant women in Bali.