ORIGINAL ARTICLE

Comparing the Effect of Human Wisdom Teeth Pulverized in Micron and Nano Particle Dimensions as Grafting Material in Healing of Tibial Bone Defect

Mostafa Govahi , Alireza Navab Azam, Seyed Hossein Tabatabai, Fatemeh Mirjalili

Mostafa Govahi
Assistant Professor and specialist in oral and maxillofacial surgery, Shahid Sadoughi Faculty of Dentistry, Yazd, Iran. Email: mostafagavahi@gmail.com

Alireza Navab Azam
Assistant Professor and specialist in oral and maxillofacial surgery, Shahid Sadoughi Faculty of Dentistry, Yazd, Iran

Seyed Hossein Tabatabai
Associate professorand specialist of oral and maxillofacial pathology, Shahid Sadoughi Faculty of Dentistry, Yazd, Iran

Fatemeh Mirjalili
Assistant Professor of Materials Engineering and nanocomposite, Azad University of Meybod, Yazd, Iran
Online First: November 23, 2016 | Cite this Article
Govahi, M., Azam, A., Tabatabai, S., Mirjalili, F. 2016. Comparing the Effect of Human Wisdom Teeth Pulverized in Micron and Nano Particle Dimensions as Grafting Material in Healing of Tibial Bone Defect. Bali Medical Journal 5(1): 185-192. DOI:10.15562/bmj.v5i1.347


Background: In this article, we decided to introduce an available, affordable and biocompatible material from human teeth using nanotechnology to repair bone defects. Totally impacted wisdom teeth of human, which had been removed by surgery, were prepared as powder in two particle sizes of 500 micron and nano (up to 100 nm) after sterilization. Method: Test cases were eight white rabbits of New Zealand species that were divided into 2 groups. Pores with 6 × 6 mm dimensions were created at hamstring area of tibia bone. In left leg tibia’s pore, nanoparticles powder and in the right leg tibia’s pore, micro particles powders were placed. The groups of two were sacrificed after 4, 8, 12 and 16 weeks. Samples underwent histomorphometric analysis and radiological analysis. The results showed the superiority of nano-groups in the percentage of new bone formation (26.62±10.88) over micro-groups (14.36±8.4) to (P-value = 0.015). Obtained Hounsfield number for micro-particle group was 2477±480 and for nanoparticle group was 1387±429 (p-value = 0.001). The differences in value soft bone vitality, inflammation, and foreign body reaction were not significant between the two groups of micro and nano. In micro particle group, despite suitable biocompatibility and Osseo integration, due to higher density and degree of crystalline, absorption and replacement rates by new bone and overall percentage of new bone formed were lower than nano group.

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