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The effect of Curcuma longa on fasting blood glucose, MMP-9 and IFN-γ in diabetes mellitus: an experimental study


Background: Turmeric (Curcuma longa) contains curcumin as an active ingredient that has ability as a potent antioxidant and antiinflammation. Turmeric extract can improve glucose level and surpress micro and microangiopathy complication in Diabetes Mellitus Type 2. The aim of this study was to investigate the role of turmeric extract administration on fasting glucose level, MMP-9, and IFN-γ.

Methods: Experimental PostTest Only Control Group Design study was conducted. Twenty four Wistar rats aged 2 months weighing 180-200 grams were adapted for 2 days, divided into 4 groups randomly: negative control group induced by STZ-NA only (P1),  positive control group induced by STZ-NA and received metformin 45mg/kgBW/day (P2), treatment group induced by STZ-NA and received  turmeric extract 200mg/kgBW/day (K1), and  ), treatment group induced by STZ-NA and received   combination of metformin 22,5mg/kgBW/day and turmeric extract 100mg/kgBW/day.  The levels of fasting glucose serum were measured by spectrophotometric method, while ELISA measured the levels of MMP-9 and IFN-γ.

Results: Mean + SD Fasting blood glucose level was decreased respectively from K1, K2, P1 and P2 (277.00 + 4.00, 100.00 + 2.00, 98.04 + 3.00, 92.00 + 3.00 mg/dl). MMP-9 level was decreased respectively from K1, K2, P1 and P2 (653.09 + 18.00, 328.00 + 14.00, 156.00 + 4.00, 131.00 + 4.00 ng/ml). IFN–γ level was decreased respectively from K1, K2, P1 and P2 (300 + 16.00, 85.00 + 3.00, 61.05 + 2.04, 49.00 + 1.09 ng/ml.) Kruskal Wallis test for Fasting blood glucose, MMP-9, dan IFN–γ shows significant differences among groups. (p=0.004, p=0.004, dan p=0.004). Using post hoc test, all groups significantly differ with p=0.000.

Conclusion: The administration of turmeric extract improves fasting glucose level, MMP-9, and IFN-γ in type 2 DM Wistar rats


  1. Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pract. 2019;157:107843.
  2. Mihardja L, Soetrisno U, Soegondo S. Prevalence and clinical profile of diabetes mellitus in productive aged urban Indonesians. J Diabetes Investig. 2014;5(5):507–12.
  3. Oguntibeju OO. Type 2 diabetes mellitus, oxidative stress and inflammation: examining the links. Int J Physiol Pathophysiol Pharmacol. 2019;11(3):45–63.
  4. Cozachenco D, Selles MC, Ribeiro FC. Interferon- γ as a potential link between diabetes mellitus and dementia. J Neurosci. 2019;39(24):4632–5.
  5. Sirisha R, Paramjyothi P. Effect of walking on Fasting Blood Sugar and HbA1c in type 2 Diabetes Mellitus. MedPulse Int J Physiol. 2019;10(3):47–9.
  6. Poolsup N, Suksomboon N, Kurnianta PDM, Deawjaroen K. Effects of curcumin on glycemic control and lipid profile in prediabetes and type 2 diabetes mellitus: A systematic review and meta-analysis. PLoS One. 2019;14(4):1–18.
  7. Guo J, Cao X, Hu X, Li S, Wang J. The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats. BMC Pharmacol Toxicol. 2020;21(1):1–10.
  8. Panahi Y, Ahmadi Y, Teymouri M, Johnston TP, Sahebkar A. Curcumin as a potential candidate for treating hyperlipidemia: A review of cellular and metabolic mechanisms. J Cell Physiol. 2018;233(1):141–52.
  9. Ali Hussain HEM. Hypoglycemic, hypolipidemic and antioxidant properties of combination of Curcumin from Curcuma longa, Linn, and partially purified product from Abroma augusta, Linn. in streptozotocin induced diabetes. Indian J Clin Biochem. 2002;17(2):33–43.
  10. Abdollahi E, Momtazi AA, Johnston TP, Sahebkar A. Therapeutic effects of curcumin in inflammatory and immune-mediated diseases: A nature-made jack-of-all-trades? Vol. 233, Journal of Cellular Physiology. 2018. 830–848 p.
  11. Hussain Z, Thu HE, Amjad MW, Hussain F, Ahmed TA, Khan S. Exploring recent developments to improve antioxidant, anti-inflammatory and antimicrobial efficacy of curcumin: A review of new trends and future perspectives. Mater Sci Eng C [Internet]. 2017;77:1316–26. Available from:
  12. Sovia E, Sukandar EY, Sigit JI, Sasongko LDN. Efek Rimpang Kunyit (Curcuma longa L.) dan Bawang Putih (Allium sativum L.) terhadap Sensitivitas Insulin pada Tikus Galur Wistar. Maj Kedokt Bandung. 2011;43(4):153–9.
  13. Saja K, Babu MS, Karunagaran D, Sudhakaran PR. Anti-inflammatory effect of curcumin involves downregulation of MMP-9 in blood mononuclear cells. Int Immunopharmacol. 2007;7(13):1659–67.
  14. Mogharrabi M, Rahimi HR, Hasanzadeh S, Dastani M, Kazemi-Oskuee R, Akhlaghi S, et al. The effects of nanomicelle of curcumin on the matrix metalloproteinase (MMP-2, 9) activity and expression in patients with coronary artery disease (CAD): A randomized controlled clinical trial. ARYA Atheroscler. 2020;16(3):1–10.
  15. Li B-Y, Tan W, Zou J-L, He Y, Yoshida S, Jiang B, et al. Role of interferons in diabetic retinopathy. World J Diabetes. 2021;12(7):939–53.
  16. Yang F, Yu J, Ke F, Lan M, Li D, Tan K, et al. Curcumin alleviates diabetic retinopathy in experimental diabetic rats. Ophthalmic Res. 2018;60(1):43–54.
  17. Gomes MB, Rathmann W, Charbonnel B, Khunti K, Kosiborod M, Nicolucci A, et al. Treatment of type 2 diabetes mellitus worldwide: Baseline patient characteristics in the global DISCOVER study. Diabetes Res Clin Pract. 2019;151:20–32.
  18. Cao L, Zhi D, Han J, Kumar Sah S, Xie Y. Combinational effect of curcumin and metformin against gentamicin-induced nephrotoxicity: Involvement of antioxidative, anti-inflammatory and antiapoptotic pathway. J Food Biochem. 2019;43(7):1–9.

How to Cite

Ovaditya, S. Z., Brilliantika, S. P. ., Chodidjah, C., & Sumarawati, T. . (2022). The effect of Curcuma longa on fasting blood glucose, MMP-9 and IFN-γ in diabetes mellitus: an experimental study. Bali Medical Journal, 11(3), 1996–2002.




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Shafira Zahra Ovaditya
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Surya Pratama Brilliantika
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Chodidjah Chodidjah
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Titiek Sumarawati
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