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The effect of kefir polysaccharide extract on the expression of matrix metalloproteinase 9 (MMP-9) and integrin activation of HeLa cervical carcinoma culture cell

  • Yahya Irwanto ,
  • Eddy Mustofa ,
  • Teguh Wiyono ,
  • Dodi Tri Oktafianto ,
  • Arif Rahman Nurdianto ,
  • Robby Rinaldi Widodo ,


Background: Cervical cancer is the leading cause of cancer in the world, especially in developing countries. Kefir is a goat's milk product that contains a lot of nutrients and bioactive complex which are involved in the synthesis of the anticancer, and have anticarcinogenic and antimutagenic effect. Integrins are involved in various biological processes associated with cancer development, such as migration, invasion, differentiation and proliferation. MMP-9 can also degrade ECM through proteolytic action, affecting cell-cell interactions in ECM. This research aimed to see the effect of kefir on MMP-9 and integrins activation. Thus, it is expected that kefir can be used as an additional supplement in patients with cervical cancer.

Methods: This research study was conducted in an experimental laboratory in vitro using HeLa cells as the object of research. Data were analyzed using the SPSS program to determine the most effective dose of kefir and a regression test to determine the effect between integrins and MMP-9.

Results: The mean value of MMP-9 and integrin at a dose of 180 µg/ml was 165.1 ± 5.8 and 378.5 ± 12.6 respectively. This result had a significant value of p = 0.0000 for both MMP-9 and integrin-b. In the regression test, the value of p = 0.05 was obtained while the coefficient value was positive (0.565).

Conclusion: The higher the expression of MMP-9, the activation of the integrin-b would increase. Kefir extract dosage of 180 µg/ml significantly reduced MMP-9 and Integrin-b expression. MMP-9 affects improving Integrin-b


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How to Cite

Yahya Irwanto, Eddy Mustofa, Teguh Wiyono, Dodi Tri Oktafianto, Arif Rahman Nurdianto, & Robby Rinaldi Widodo. (2022). The effect of kefir polysaccharide extract on the expression of matrix metalloproteinase 9 (MMP-9) and integrin activation of HeLa cervical carcinoma culture cell. Bali Medical Journal, 11(2), 803–806.




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