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Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn

Abstract

Background: Purnajiwa is one of the medicinal plants in Bali. Species information regarding this plant is still unconfirmed. Currently, this plant is considered rare and endangered. Molecular identification supports authentication at the species level, which will also support conservation efforts.

Method: Molecular identification using DNA Barcoding was carried out on purnajiwa from three sampling locations in Bali (Jimbaran, Mambal, and Bedugul) using rbcL primers (rbcLaF and rbcLaR). Data analysis was performed using BLAST with species on GenBank. Pairwise and similarity values ​​were used to measure the three samples' proximity to the Gen Bank species.

Result: The phylogenetic tree was constructed with the Maximum Likelihood and Tamura-3-parameter model. The analysis results show a close relationship between the purnajiwa from Jimbaran and Mambal with the species Kopsia arborea (KP095079). Meanwhile, the Purnajiwa from Bedugul showed a difference from the other two purnajiwa and was closely related to Euchresta horsfieldii (U74225).

Conclusion: It can be concluded that the Purnajiwa from Jimbaran and Mambal is a species of Kopsia arborea while the Purnajiwa from Bedugul is a species of Euchresta horsfieldii.

References

  1. Ariati PEP, Wirawan IGP, Sasadara MMV. Optimization of primer and polymerase chain reaction conditions to amplify COI locus for identification of Purnajiwa (Euchresta horsfieldii (Lesch.) Benn.) collected from Bedugul, Bali. IOP Conf Ser Earth Environ Sci. 2021;913(1).
  2. Priyadi A, Feng C, Kang M, Huang H. Development of 10 single-copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant. Appl Plant Sci. 2018;6(9):10–3.
  3. Chen XD, Hu J, Li JX, Chi FS. Cytotoxic monoterpenoid indole alkaloids from the aerial part of Kopsia arborea. J Asian Nat Prod Res [Internet]. 2020;22(11):1024–30. Available from: https://doi.org/10.1080/10286020.2019.1680646
  4. Prihantini AI, Krisnawati, Ali Setyayudi. Antioxidant and alpha-glucosidase inhibitory activities of Euchresta horsfieldii. Biofarmasi J Nat Prod Biochem. 2019;17(2):61–4.
  5. Apriliani RT, Wirawan IGP, Adiartayasa W. Phytochemical Analysis And Antioxidant Activity Of Purnajiwa Fruit Extract (Euchresta horsfieldii (Lesch.) Benn. Int J Biosci Biotechnol. 2020;8(1):31.
  6. Krishnamurthy PK, Francis RA. A critical review on the utility of DNA barcoding in biodiversity conservation. Biodivers Conserv. 2012;21(8):1901–19.
  7. Wirawan IGP, Malida M, Sasadara V, Wijaya IN. DNA barcoding in molecular identification and phylogenetic relationship of beneficial wild Balinese red algae , Bulung sangu ( Gracilaria sp .). Bali Med J. 2021;10(1):82–8.
  8. Kim D yoon, Kim B mi, Park T yoon S, Cho G, Kim T woo, Shin S. First record of Teleogryllus ( Brachyteleogryllus ) marini Otte & Alexander , 1983 ( Orthoptera : Gryllidae ) in korea and discussion of its continued misidentification using DNA barcoding. J Asia Pac Entomol [Internet]. 2022;25(3):101959. Available from: https://doi.org/10.1016/j.aspen.2022.101959
  9. Xin T, Li R, Lou Q, Lin Y, Liao H, Sun W, et al. Phytomedicine Application of DNA barcoding to the entire traditional Chinese medicine industrial chain : A case study of Rhei Radix et Rhizoma. Phytomedicine [Internet]. 2022;105(August):154375. Available from: https://doi.org/10.1016/j.phymed.2022.154375
  10. Chen S, Pang X, Song J, Shi L, Yao H, Han J, et al. A renaissance in herbal medicine identification: From morphology to DNA. Biotechnol Adv. 2014;32(7):1237–44.
  11. Sartori AG de O, Cesar ASM, Woitowicz FCG, Saliba ASMC, Ikegaki M, Rosalen PL, et al. Plant genetic diversity by DNA barcoding to investigate propolis origin. Phytochemistry. 2022;200(February):1–11.
  12. Alberts PSF, Meyer JJM. Integrating chemotaxonomic-based metabolomics data with DNA barcoding for plant identification: A case study on south-east African Erythroxylaceae species. South African J Bot [Internet]. 2022;146:174–86. Available from: https://doi.org/10.1016/j.sajb.2021.10.005
  13. CBOL. A DNA mini-barcode for land plants. Mol Ecol Resour. 2014;14(3):437–46.
  14. von Cräutlein M, Korpelainen H, Pietiläinen M, Rikkinen J. DNA barcoding: A tool for improved taxon identification and detection of species diversity. Biodivers Conserv. 2011;20(2):373–89.
  15. Miao L, Xi-Wen L, Bao-Seheng L, Lu L, Yue-Ying R. Species identification of poisonous medicinal plant using DNA barcoding. Chin J Nat Med [Internet]. 2019;17(8):585–90. Available from: http://dx.doi.org/10.1016/S1875-5364(19)30060-3
  16. Silalahi D, Wirawan IGP, Sasadara MMV. Optimization of annealing temperature for amplification of EhoscnOla locus in pranajiwa (Euchresta horsfieldii) plant collected from mountains, urban and coastal areas in Bali. IOP Conf Ser Earth Environ Sci. 2021;913(1).
  17. Khang DOTAN, Huy TGIA, Pham N, Thi ANH, Thi D, Quyen H, et al. Genetic diversity of Burmese grape ( Baccaurea ramiflora Lour .) cultivars and Ha Chau cultivar identification based on DNA barcodes. 2022;23(7):3513–20.
  18. Hebert PDN, Cywinska A, Ball SL, DeWaard JR. Biological identifications through DNA barcodes. Proc R Soc B Biol Sci. 2003;270(1512):313–21.
  19. Hubert N, Hanner R. DNA Barcoding, species delineation and taxonomy: a historical perspective. DNA Barcodes. 2016;3(1):44–58.
  20. Liu J, Jiang J, Song S, Tornabene L, Chabarria R, Naylor GJP, et al. Multilocus DNA barcoding - Species Identification with Multilocus Data. Sci Rep. 2017;7(1):1–12.
  21. Nijman V, Aliabadian M. DNA barcoding as a tool for elucidating species delineation in wide-ranging species as illustrated by owls (Tytonidae and Strigidae). Zoolog Sci. 2013;30(11):1005–9.
  22. Čandek K, Kuntner M. DNA barcoding gap: Reliable species identification over morphological and geographical scales. Mol Ecol Resour. 2015;15(2):268–77.
  23. Shinwari ZK, Jan SA, Khalil AT, Khan A, Ali M, Qaiser M, et al. Identification and phylogenetic analysis of selected medicinal plant species from Pakistan: DNA barcoding approach. Pakistan J Bot. 2018;50(2):553–60.
  24. Chen Y, Wang B, Chen J, Wang X, Wang R, Peng S, et al. Identification of rubisco rbcL and rbcS in Camellia oleifera and their potential as molecular markers for selection of high tea oil cultivars. Front Plant Sci. 2015;6(MAR):1–11.
  25. Wattoo JI, Saleem MZ, Shahzad MS, Arif A, Hameed A. DNA Barcoding: Amplification and sequence analysis of rbcl and matK genome regions in three divergent plant species. J Biol Sci. 2016;4(1):3–7.

How to Cite

Putu Eka Pasmidi Ariati, Sasadara, M. M. V., I Gede Putu Wirawan, Made Sritamin, I Ketut Suada, I Nyoman Wijaya, Rindang Dwiyani, I Putu Sudiarta, & Ida Ayu Putri Darmawati. (2022). Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn. Bali Medical Journal, 11(3), 1681–1685. https://doi.org/10.15562/bmj.v11i3.3815

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Putu Eka Pasmidi Ariati
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Maria Malida Vernandes Sasadara
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I Gede Putu Wirawan
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Made Sritamin
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I Ketut Suada
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I Nyoman Wijaya
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Rindang Dwiyani
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I Putu Sudiarta
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Ida Ayu Putri Darmawati
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