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The pattern of osteocyte in dental socket bone regenerative induced by hydroxyapatite bovine tooth graft

  • Nanik Zubaidah ,
  • Sri Kunarti ,
  • Nadira Nurin Febrianti ,
  • Arif Rahman Nurdianto ,
  • Wanda Oktaria ,
  • Muhammad Luthfi ,

Abstract

Background: Tooth extraction is a procedure with the highest prevalence of causing alveolar bone damage. After extraction, the dimension will change. This procedure needs a balance of bone formation and resorption. Osteocytes are the most common cells in bone and have an essential role in bone formation. This study explores the effect of bovine tooth grafts on the number of osteocytes in the socket preservation process post-dental extraction.

Methods: A total of 28 Wistar rats, 14 control and 14 treatment groups, were extracted on the lower left incisor. Control group with PEG without induction and treatment group with bovine tooth graft and PEG induction. On the 14th and 28th days, the rats were euthanized. Then perform histological examination with HE staining and cell counting using a light microscope

Results:  We found the treatment group had higher the average number of the osteocyte, which was 146.43±11.41 on 14th day and 288.14±161.91 on 28th day. There was a significant increase in osteocytes among the treatment group compared to the control (p=0.000).

Conclusion: Bovine tooth graft application as dental socket preservation was proven to increase the number of osteocyte cells in the alveolar bone of Wistar rats after extraction. Bovine tooth grafts as dental socket preservation materials increased the number of osteocytes at a peak at 28 days.

References

  1. Goswami A, Ghorui T, Bandyopadhyay R, Sarkar A, Ray A. A General Overview of Post Extraction Complications-Prevention, Management and Importance of Post Extraction Advices. Fortune J Heal Sci. 2020;03(03):135-147. doi:10.26502/fjhs014
  2. Fan G, Shen Y, Cai Y, Zhao J hong, Wu Y. Uncontrollable bleeding after tooth extraction from asymptomatic mild haemophilia patients: two case reports. BMC Oral Health. 2022;22(1):1-8. doi:10.1186/s12903-022-02074-9
  3. Moran IJ, Richardson L, Heliotis M, Bewick A. A bleeding socket after tooth extraction. BMJ. 2017;357(box 1):1-5. doi:10.1136/bmj.j1217
  4. Nardiatmo SPS, Mapangara S, Jais AI. Socket preservation after tooth extraction : a systematic review Mempertahankan soket setelah pencabutan gigi : tinjauan sistematik. Makassar Dent J. 2019;8(2):91-96.
  5. Van Der Weijden F, Dell’Acqua F, Slot DE. Alveolar bone dimensional changes of post-extraction sockets in humans: A systematic review. J Clin Periodontol. 2009;36(12):1048-1058. doi:10.1111/j.1600-051X.2009.01482.x
  6. Fee L. Socket preservation. Br Dent J. 2017;222(8):579-582. doi:10.1038/sj.bdj.2017.355
  7. Listari KM, Ruhadi I, Ulfa N. Ekspresi Rankl Pada Defek Tulang Dengan Pemberian Xenograft Dibandingkan Dengan Xenograft Dan Prf. E-Prodenta J Dent. 2019;3(1):216-224. doi:10.21776/ub.eprodenta.2019.003.01.5
  8. Lobb DC, DeGeorge BR, Chhabra AB. Bone Graft Substitutes: Current Concepts and Future Expectations. J Hand Surg Am. 2019;44(6):497-505.e2. doi:10.1016/j.jhsa.2018.10.032
  9. Ratnayake JT, Ross ED, Dias GJ, et al. Preparation, characterisation and in-vitro biocompatibility study of a bone graft developed from waste bovine teeth for bone regeneration. Mater Today Commun. 2020;22:100732. doi:10.1016/j.mtcomm.2019.100732
  10. Tanaka JLO, Medici Filho E, Salgado JAP, et al. Comparative analysis of human and bovine teeth: Radiographic density. Braz Oral Res. 2008;22(4):346-351. doi:10.1590/S1806-83242008000400011
  11. Yassen GH, Platt JA, Hara AT. Bovine teeth as a substitute for human teeth in dental research: a review of the literature. J Oral Sci. 2011;53(3):273-282. doi:10.2334/josnusd.53.273
  12. Larasati D, Sulistiani R. Kajian Efek Analgetik Dispersi Padat Ibuprofen - PEG 6000 Menggunakan Metode Writhing Test. J Kesehat Madani Med. 2020;11(01):35-43.
  13. Hartatiek, Yudyanto, Nada Shofura F, et al. Morphology, porosity, and biodegradation of PVA/CS/PEG/HaP nanofiber composites as a scaffold in bone tissue engineering. AIP Conf Proc. 2020;2231(April):2-8. doi:10.1063/5.0002678
  14. Nugraha B. Application of PEG in Drug Delivery System. 2016;(January 2016):137-147. doi:10.1142/9789813140417_0006
  15. Hassan AA, AL-Ghaban NM. Histological Evaluation of the Effect of Local Application of Grape Seed Oil on Healing Process of Extracted Tooth Socket in Rabbits. Diyala J Med. 2019;17(2):70-84. doi:10.26505/djm.17024670515
  16. Chairunas, Saputri D, Putri MK. Daya Fitro-respon Ekstrak Etanol Daun Kelor (Moringa oleifera) Terhadap Sel Osteosit dan Matriks Tulang Mandibula Tikus (Rattus norvegicus). Cakradonya Dent J. 2020;12(2):83-88.
  17. Prahasanti C, Nugraha AP, Saskianti T, Suardita K, Riawan W, Ernawati DS. Exfoliated human deciduous tooth stem cells incorporating carbonate apatite scaffold enhance BMP-2, and BMP-7 and attenuate MMP-8 expression during initial alveolar bone remodelling in Wistar rats (Rattus norvegicus). Clin Cosmet Investig Dent. 2020;12(November):79-85. doi:10.2147/CCIDE.S245678
  18. Khoswanto C. A New Technique for Research on Wound Healing through Extraction of Mandibular Lower Incisors in Wistar Rats. Eur J Dent. 2019;13(2):235-237. doi:10.1055/s-0039-1694312
  19. Sadr K, Aghbali A, Sadr M, Abachizadeh H, Azizi M, Mesgari Abbasi M. Effect of Beta-Blockers on Number of Osteoblasts and Osteoclasts in Alveolar Socket Following Tooth Extraction in Wistar Rats. J Dent (Shiraz, Iran). 2017;18(1):37-42.
  20. Zubaidah N, Adventa Y, Pratiwi DD, Mooduto L, Setiawati EM, Kunarti S. The Pattern of Collagen, Col1A, BSP and MMP-8 in Alveolar Bone Socket Post Tooth Extraction of Rattus Norvegicus Strain Wistar After Induced with Hydroxyapatite Bovine Tooth Graft. J Int Dent Med Res. 2021;14(4):1447-1452.
  21. Sari DS, Maduratna E, Ferdiansyah, et al. Osteogenic Differentiation and Biocompatibility of Bovine Teeth Scaffold with Rat Adipose-derived Mesenchymal Stem Cells. Eur J Dent. 2019;13(2):206-212. doi:10.1055/s-0039-1694305
  22. Kresnoadi U, Rahayu RP, Rubianto M, Sudarmo SM, Budi HS. TLR2 signalling pathway in alveolar bone osteogenesis induced by Aloe vera and xenograft (XCB). Braz Dent J. 2017;28(3):281-286. doi:10.1590/0103-6440201600834
  23. Vijayan A, Sabareeswaran A, Kumar GSV. PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing. Sci Rep. 2019;9(1):1-12. doi:10.1038/s41598-019-55214-7
  24. Olaitan OH, Komolafe OA, Owotade FJ, Saka OS. Histologic Assessment of Extraction Sockets Following Tooth Extraction : Suitability of a Rabbit Model. Niger J Dent Res. 2019;4(1):1-4.
  25. Chul Ho Jang, Cho GW, Song A-J. Effect of Bone Powder / Mesenchymal Stem Cell / BMP2 / Fibrin Glue on Osteogenesis in a Mastoid Obliteration Model. In Vivo (Brooklyn). 2020;34:1103-1110. doi:10.21873/invivo.11881
  26. Sari RP, Kurniawan H. Effectiveness of Anadara granosa shell-Stichopus hermanni granules at accelerating woven bone formation fourteen days after tooth extraction. Dent J (Majalah Kedokt Gigi). 2019;52(4):177. doi:10.20473/j.djmkg.v52.i4.p177-182
  27. Blair HC, Larrouture QC, Li Y, et al. Osteoblast differentiation and bone matrix formation in vivo and in vitro. Tissue Eng - Part B Rev. 2017;23(3):268-280. doi:10.1089/ten.tub.2016.0454
  28. Musson DS, Gao R, Watson M, et al. Bovine bone particulates containing bone anabolic factors as a potential xenogenic bone graft substitute. J Orthop Surg Res. 2019;14(1):1-11. doi:10.1186/s13018-019-1089-x
  29. Rupani A, Balint R, Cartmell SH. Osteoblasts and their applications in bone tissue engineering. Cell Health Cytoskeleton. 2012;4:49-61. doi:10.2147/CHC.S21845
  30. Anisah A, Delina M, Aisah N, Gustiono D. Pembuatan Graft Tulang Dengan Proses Ekstraksi Senyawa Hidroksiapatit Dari Tulang Korteks Sapi. Spektra J Fis dan Apl. 2018;3(1):31-36. doi:10.21009/spektra.031.05
  31. Kattimani VS, Kondaka S, Lingamaneni KP. Hydroxyapatite–-Past, Present, and Future in Bone Regeneration. Bone Tissue Regen Insights. 2016;7:BTRI.S36138. doi:10.4137/btri.s36138
  32. Huntley R, Jensen E, Gopalakrishnan R, Mansky KC. Bone morphogenetic proteins: Their role in regulating osteoclast differentiation. Bone Reports. 2019;10(September 2018). doi:10.1016/j.bonr.2019.100207
  33. Graunaite I, Lodiene G, Maciulskiene V. Pathogenesis of Apical Periodontitis: a Literature Review. J Oral Maxillofac Res. 2011;2(4):1-15. doi:10.5037/jomr.2011.2401

How to Cite

Zubaidah, N., Kunarti, S. ., Febrianti, N. N. ., Nurdianto, A. R. ., Oktaria, W. ., & Luthfi, M. . (2022). The pattern of osteocyte in dental socket bone regenerative induced by hydroxyapatite bovine tooth graft. Bali Medical Journal, 11(3), 1489–1493. https://doi.org/10.15562/bmj.v11i3.3844

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