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Regenerative alveolar bone in dental sockets of diabetic wistar rats post tooth extraction


Background: The bone healing of the alveolar socket post-dental extraction is critical in dental services. Delayed healing is a common problem in diabetes mellitus (DM) patients. It is due to the impaired angiogenic response and microvascular complications. Okra fruit extract can control diabetes mellitus (DM) in several ways, delaying glucose absorption and regenerating pancreatic cells. It can impact an increase in insulin secretion and glycogenesis to control hyperglycemia in DM. This study analyzed the regenerative alveolar bone through the expression of TGF-β2 in the tooth socket of Wistar rats with diabetes mellitus post-extraction after administration of okra fruit extract.

Patients and methods: Extraction of the rat mandibular left incisor was performed using a pair of modified forceps. The tooth sockets were then rinsed using a saline solution. Two groups (control & treatment) of four rats were sacrificed on days 3, 5, and 7. The socket tissues from the rats were then immunohistochemically analyzed

Results: The average level of TGF‐β1 expression in the treatment (T) groups was higher compared to the control (C) group: day 3 (10.50±2.67 versus 4.83±1.17), day 5 (12.33±1.63 versus 5.17±2.32), and day 7 (13.00±2.83 versus 4.50±1.05), with P = 0.000

Conclusion: The administration of okra fruit extract can increase regenerative alveolar bone healing through increased expression of TGF-β2 in the dental socket after tooth extraction of diabetic Wistar rats.


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How to Cite

Nanik Zubaidah, Muhammad Luthfi, Wisnu Setyari, Retno Palupi, Nur Imamatul Ummah, Ardyta Lintang Maheswari, Arif Rahman Nurdianto, & Fathillah Abdul Razak. (2022). Regenerative alveolar bone in dental sockets of diabetic wistar rats post tooth extraction. Bali Medical Journal, 11(3), 1699–1705.




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Nanik Zubaidah
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Muhammad Luthfi
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Wisnu Setyari
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Retno Palupi
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Nur Imamatul Ummah
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Ardyta Lintang Maheswari
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Arif Rahman Nurdianto
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Fathillah Abdul Razak
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