Background: Skin graft is one of the best options for wound closure. This method is the gold standard for skin burns and large open wounds. Coffee, or Robusta coffes (BP-2) is believed to reduce the level of hyperpigmentation due to chlorogenic acid, a major polyphenolic found in coffee with anti-tyrosinase activity. However, the anti-tyrosinase activity of BP-42 coffee extract is unknown. This study aims to measure the chlorogenic acid content of BP-42 coffee bean extract and its anti-tyrosinase activity.
Methods: BP-42 coffee beans were extracted using ethanol by maceration technique. Then, the thin-layer chromatography (TLC) profile of the BP-42 coffee bean extract was made to determine the chlorogenic acid content. At the same time, its anti-tyrosinase activity was performed using the spectrophotometric method. The chlorogenic acid's content was determined using the TLC-densitometric method. Data were analyzed using SPSS version 20.0 for Windows.
Results: BP-42 coffee bean extract had chlorogenic acid concentrations at 12.452 mg/g extract. As expected, the extract exhibited anti-tyrosinase activity with IC50 at 312.213 ppm, while kojic acid showed higher bioactivity (IC50 = 30.696 ppm).
Conclusion: BP-42 coffee bean extract showed a notable anti-tyrosinase activity, promising to be used as the natural anti-pigmentation candidate.