ORIGINAL ARTICLE

Effect of Virgin Coconut Oil Compared to Corn Oil in World Health Organization Formula on Malondialdehyde Expression in Intestine of Severe Acute Malnutrition Wistar Rat

I Gusti Ayu Putu Eka Pratiwi

I Gusti Ayu Putu Eka Pratiwi
Department of Child Health, Faculty of Medicine, Udayana University/Sanglah Hospital, Bali-Indonesia. Email: ayuka73@yahoo.com
Online First: February 08, 2017 | Cite this Article
Eka Pratiwi, I. 2017. Effect of Virgin Coconut Oil Compared to Corn Oil in World Health Organization Formula on Malondialdehyde Expression in Intestine of Severe Acute Malnutrition Wistar Rat. Bali Medical Journal 6(1): 73-75. DOI:10.15562/bmj.v6i1.403


Background: In severe acute malnutrition, oxidative stress is occurred and it cause dysfunction of various tissue, including intestine. Intestinal function is impaired and there is altered epitheal transport. It may be related with intestinal oxidative stress. Virgin coconut oil contain a lot of antioxidant properties, could be solved that problem. Methods: This study aimed to investigate the oxidative stress in intestine after given virgin coconut oil compared to corn oil in rehabilitation stage of severe acute malnutrition treatment of wistar rat. A posttest only control group design experimental study was done to investigate effect of VCO compare to corn oil on malondialdehyde (MDA) expression in intestine in male wistar rat with severe acute malnutrition. Thirty eight severe acute malnutrition male wistar rats were used in this study, which was divided by two groups, Group A was rat feeded by Formula WHO  (F75 and F100) that contain VCO, Group B was rat feeded Formula WHO that contain corn oil. This formula was taken for 28 days. Results: At the end of this study, one rat was died (Group B), and 37 rats were analysed for MDA expression in intestine. Mean of MDA expression in Group A was 1.45% (SD = 0.70 %) and Group B was 1.51% (SD = 0.54%).  There was no mean differences for MDA expression between Group A and Group B (p for T-test was 0,71, mean difference -0.06 (95% CI = -0.48-0.36). Conclusion: In rehabilitation phase of treatment of severe acute malnutrition wistar rat, there is no differences in MDA expression in intestine between using VCO and corn oil.

 

References

World Health Organization. 2013. Guideline Updates on the Management of Severe Acute Malnutrition in Infants and Children. http://www.apps.who.int>iris>9789241506328_eng

Pelissier, M.A., Bourdet, N., Marques-Dossou, F., Desjeux H.F., Albrecht, R. 2002. Palm Versus Sybean Oil on Intestinal Recovey from Malnutrition in Guinea Pigs. Pediatr Res, 52:119-24.

Darmon, N., Pelissier, M.A., Heyman, M., Albrecht, R., Desjeux, J.E. 1993. Oxidative Stress may Contribute to the Intestinal Dysfuction of Weanling Rats Fed a Low Protein Diet. J Nutr, 123 (6):1068-75

Patterson, E., Wall, R., Fitzgerald, G.F., Ross, R.P., Stanton, C. 2012. Health Implication of High Dietary Omega-6 Polyunsaturated Fatty Acids. Journal of Nutrition and Metabolism, 2012:1-16.

Yeap, S.K., Beh, B.K., Ali, N.M., Yusof, H.M., Ho, W.Y., Koh, S.P., Alitheen, N.B., Long., K. 2015. Antistress and antioxidant effects of virgin coconut oil in vivo. Experimental and Therapeutic Medicine, 9:39-42.

Kementerian Kesehatan Republik Indonesia. 2013. Petunjuk Teknis Tata Laksana Anak Gizi Buruk. Edisi revisi. Jakarta: Departemen Kesehatan RI.

Caglar, O.M., Bilgihan, A., Turkcu, U.O., Biberoglu, G., Take, G. 2012. The Effect of Zinc on Ethanol-induced Oxidative Stress in Rat Liver. Turk J Biochem, 37(4):437-44.

Ghone, R.A., Suryakar, A.N., Kulhalli, P.M., Bhagat, S.S., Padalkar, R.K., Karnik, A.C., Hundekar, P.S., Sangle, D.A. 2013. A Study of Oxidative Stress Biomarkers and Effect of Oral Antioxidant Supplementation in Severe Acute Malnutrition. Journal of Clinical and Diagnostic Research, 7(10): 2146-8.

Bandsma, R.H.J., Spoelstra, M.N., Mari, A., Mendel, M., Rheenen, P.F., Senga, E., Dijk, T., Heikens, G.T. Impaired Glucose Absorption in Children with Severe Malnutrition . The Journal of Pediatrics, 158(2):282-7.

Nieto, N., Mesa, M.D., Lopez-Pedrosa, J.M., Torres, M.I., Rios, A., Suarez, M.D., Gil, A. 2007. Contribution of Polyunsaturated Fatty Acids to Intestinal Repair in Protein-Energy Malnutrition. Digestive Diseases and Sciences, 52(6):1485-96.

Iritani, N., Ikeda, Y. 1982. Activation of Catalase and Other Enzymes by Corn Oil Intake. J. Nutr., 112: 2235-9.

Eka-Pratiwi, I., Soetjiningsih, S., Mantik-Astawa, I., Trisna Windiani, I. 2016. Virgin coconut oil compared to corn oil in world health organization formula on glutathione, TNF-α, and body weight gain in severe malnourished Wistar rat Bali Medical Journal 5(3): 99-103. DOI:10.15562/bmj.v5i3.321


No Supplementary Material available for this article.
Article Views      : 0
PDF Downloads : 0