ORIGINAL ARTICLE

Eicosapentaenoic acid and docosahexaenoic acid in fish oil capsule supplementation in obese children decreases serum interleukin-6 and hepcidin and improves iron status

I Gusti Lanang Sidiartha , I Made Bakta, I Made Wiryana, I Wayan Putu Sutirtayasa, Damayanti R Sjarif

I Gusti Lanang Sidiartha
Department of Child Health, School of Medicine, Udayana University/Sanglah General Hospital, Denpasar, Bali, Indonesia. Email: lanangsidiartha@yahoo.com

I Made Bakta
Internal Medicine School of Medicine, Udayana University/Sanglah General Hospital, Denpasar, Bali, Indonesia

I Made Wiryana
Anesthesiology Department School of Medicine, Udayana University/Sanglah General Hospital, Denpasar, Bali, Indonesia

I Wayan Putu Sutirtayasa
Department of Biochemistry School of Medicine, Udayana University/Sanglah General Hospital, Denpasar, Bali, Indonesia

Damayanti R Sjarif
Departmet of Child Health University of Indonesia
Online First: February 18, 2017 | Cite this Article
Sidiartha, I., Bakta, I., Wiryana, I., Sutirtayasa, I., Sjarif, D. 2017. Eicosapentaenoic acid and docosahexaenoic acid in fish oil capsule supplementation in obese children decreases serum interleukin-6 and hepcidin and improves iron status. Bali Medical Journal 6(1): 97-101. DOI:10.15562/bmj.v6i1.410


Background: Chronic low-grade inflammation in obese children causes iron restriction, resulting in hypoferremia. Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA), active metabolites of long chain n-3 polyunsaturated fatty acid have an anti-inflammatory effect. Objective: We investigated the effect EPA and DHA in fish oil capsule supplementation on inflammation and iron status in obese children. Methods: A double-blind randomized controlled trial was conducted among 70 obese children aged six to ten years old. Thirty-five obese children received a fish oil capsule containing 45 mg EPA and 225 mg DHA (the supplemented group) and the others a virgin coconut oil capsule (the control group) for eight weeks, respectively. The changes in IL-6 and Hepcidin levels and iron status before and after supplementation were analyzed using MANCOVA test (α=0.05). Results: The study indicates a significant decrease of serum IL-6 by 1.8 pg/ml (95%CI 0.5-3.1 p=0.005), Hepcidin 9.6 nmol/l (95%CI 5.1-14.0 p=0.0001), and ferritin 10.2 ng/dl (95%CI 0.9-19.6 p=0.032). In addition, after the supplementation, we found an increase in serum iron by 13.6 mg/dl (95%CI 4.1-23.2 p=0.006) and transferrin saturation by 4.6% (95%CI 1.7-7.6 p=0.002). Conclusion: Supplementation with fish oil capsule containing EPA and DHA in obese children improved the iron status through decreasing serum IL-6 and Hepcidin.

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References

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