Skip to main content Skip to main navigation menu Skip to site footer

The effect of Curcuma longa extract on fibroblast count and collagen density in intestinal anastomosis: an experimental study on New Zealand rabbits

  • Sarwendah Pratiwi Budiman ,
  • I Gusti Bagus Adria Hariastawa ,
  • Fendy Matulatan ,

Abstract

Background: Anastomotic leakage was the most feared complication after intestinal surgery because it could bring morbidity and mortality. Curcuma longa (turmeric) has been known for its effect on wound healing by increasing fibroblast count and collagen density. Nevertheless, its effect on intestine anastomosis has not yet been proven. This study was an experimental study on New Zealand rabbits to analyze the effect of Curcuma longa extract on fibroblast count and collagen density in intestinal anastomosis.

Methods: This study was a randomized control trial experimental study on a New Zealand white rabbit. The rabbits were divided into 2 groups randomly, the control group and the treatment group, which were given Curcuma longa extract for 4 days after laparotomy intestinal resection and anastomosis. Fibroblast count and collagen density of the anastomotic area were evaluated on the 5th day after the surgery.

Results: 36 New Zealand white rabbits underwent laparotomy intestinal resection and anastomosis, then randomly divided into 2 groups. On the 5th day, the subjects were evaluated. The analysis results showed that the control group had a lower mean count of fibroblasts (9.89) than the treatment group (27.11), with the Mann-Whitney analysis showing significant differences (p-value 0.000). The lowest score for collagen density in the control group was +2, with the highest being +3. While on the treatment group, the lowest score was +3, and the highest was +4. The mean value was lower in the control group (10.61) rather than in the treatment group (26.39), with the Mann-Whitney analysis showing significant differences (p-value 0.000).

Conclusion: Curcuma longa extract had an effect on intestinal anastomosis by increasing fibroblast count and collagen density.

References

  1. Holmer C, Praechter C, Mecklenburg L, Heimesaat M, Rieger H, Pohlen U. Anastomotic stability and wound healing of colorectal anastomoses sealed and sutured with a collagen fleece in a rat peritonitis model. Asian J Surg. 2014;37(1):35-45. doi:10.1016/j.asjsur.2013.07.008.
  2. Lyra Junior HF, Rodrigues IK, Schiavon LL, D Acâmpora AJ. Ghrelin and gastrointestinal wound healing. A new perspective for colorectal surgery. Acta Cir Bras. 2018;33(3):282-294. doi:10.1590/s0102-865020180030000010.
  3. Akbik D, Ghadiri M, Chrzanowski W, Rohanizadeh R. Curcumin as a wound healing agent. Life Sci. 2014;116(1):1-7. doi:10.1016/j.lfs.2014.08.016.
  4. Erick BY, Ariandi S, Marjono DW, and Edwin D. The effect of topical curcumin extract on fibroblast count and collagen density as an indicator on accelerating clean wound healing process: a study on wistar rats. SRP. 2020; 11(5): 567-570. doi:10.31838/srp.2020.5.74.
  5. Kaur N, Garg T, Goyal AK, Rath G. Formulation, optimization and evaluation of curcumin-β-cyclodextrin-loaded sponge for effective drug delivery in thermal burns chemotherapy. Drug Deliv. 2016;23(7):2245-2254. doi:10.3109/10717544.2014.963900.
  6. Emiroglu G, Ozergin Coskun Z, Kalkan Y, et al. The Effects of Curcumin on Wound Healing in a Rat Model of Nasal Mucosal Trauma. Evid Based Complement Alternat Med. 2017;2017:9452392. doi:10.1155/2017/9452392.
  7. Novriansyah R. The Difference of Collagen Density Around Wistar Mice Wound Incision Dressing with Conventional Gauze and Occlusive Hydrocolloid for 2 and 14 days. Thesis Univ Diponegoro. 2008. p1-87.
  8. Dei Cas M, Ghidoni R. Dietary Curcumin: Correlation between Bioavailability and Health Potential. Nutrients. 2019;11(9):2147. Published 2019 Sep 8. doi:10.3390/nu11092147.
  9. Tung BT, Nham DT, Hai NT, and Thu DK. Curcuma longa, the Polyphenolic Curcumin Compound and Pharmacological Effects on Liver. Dietary Interventions in Liver Disease. 2019. p125–134. https://doi.org/10.1016/B978-0-12-814466-4.00010-0.
  10. Sahebkar A, Cicero AFG, Simental-Mendía LE, Aggarwal BB, Gupta SC. Curcumin downregulates human tumor necrosis factor-α levels: A systematic review and meta-analysis ofrandomized controlled trials. Pharmacol Res. 2016;107:234-242. doi:10.1016/j.phrs.2016.03.026.
  11. Merrell JG, McLaughlin SW, Tie L, Laurencin CT, Chen AF, Nair LS. Curcumin-loaded poly(epsilon-caprolactone) nanofibres: diabetic wound dressing with anti-oxidant and anti-inflammatory properties. Clin Exp Pharmacol Physiol. 2009;36(12):1149-1156. doi:10.1111/j.1440-1681.2009.05216.x.
  12. Petroll WM, Cavanagh HD, Barry P, Andrews P, Jester JV. Quantitative analysis of stress fiber orientation during corneal wound contraction. J Cell Sci. 1993;104 ( Pt 2):353-363. doi:10.1242/jcs.104.2.353.
  13. Gopinath D, Ahmed MR, Gomathi K, Chitra K, Sehgal PK, Jayakumar R. Dermal wound healing processes with curcumin incorporated collagen films. Biomaterials. 2004;25(10):1911-1917. doi:10.1016/s0142-9612(03)00625-2.

How to Cite

Budiman, S. P., Hariastawa, I. G. B. A., & Matulatan, F. . (2023). The effect of Curcuma longa extract on fibroblast count and collagen density in intestinal anastomosis: an experimental study on New Zealand rabbits. Bali Medical Journal, 12(1), 683–685. https://doi.org/10.15562/bmj.v12i1.4151

HTML
9

Total
2

Share

Search Panel

Sarwendah Pratiwi Budiman
Google Scholar
Pubmed
BMJ Journal


I Gusti Bagus Adria Hariastawa
Google Scholar
Pubmed
BMJ Journal


Fendy Matulatan
Google Scholar
Pubmed
BMJ Journal