Histopathological findings of the liver and kidneys of wistar rat exposed to insect repellent

Ary Andini; Lysa Veterini; Edza Aria Wikurendra; Achmad Syafiuddin; Defi Rimasari; Munifa Nur Aida

doi:10.15562/bmj.v12i2.4315

Histopathological findings of the liver and kidneys of wistar rat exposed to insect repellent

Ary Andini ,

Lysa Veterini ,

Edza Aria Wikurendra ,

Achmad Syafiuddin ,

Defi Rimasari ,

Munifa Nur Aida ,

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DOI: https://doi.org/10.15562/bmj.v12i2.4315 |
Published: 2023-04-26

Abstract

Introduction: The active substance of pyrethroids, especially dimefluthrin, in insect repellent not only plays a role in killing mosquitoes but is also toxic to human health. This study aims to analyze the effect of insect repellent exposure on the histopathological findings in the liver and kidneys of the Wistar rat.

Methods: This experimental study used 24 male wistar rats, which were divided into four groups, namely control, type of insect repellent, which included mat electric (dimefluthrin 0.566%), liquid electric (dimefluthrin 0.014%), and coil (dimefluthrin 0.031%), with a duration of exposure a day for 8 hours in 20 days.

Results: There was a significant effect of the type of insect repellent exposure on the histopathological findings of the liver and kidneys (p<0.05). This study also showed the highest rate of cell degeneration and necrosis in the liver and kidneys belonged to Mat electric repellent exposure in the liver and kidney. Higher concentrations of dimefluthrin in insect repellent have a significant effect on the histopathology of the liver and kidney.

Conclusions: The use of mosquito coils, electric mats, and liquid electricity for 8 hours over 20 days affects the histopathological evaluation of the liver and kidneys. The higher the concentration of dimetfluthrin in insect repellent, the higher the level of damage.

References

Al-Mamun MA, Ataur Rahman M, Habibur Rahman M, Hoque KMF, Ferdousi Z, Matin MN, et al. Biochemical and histological alterations induced by the smoke of allethrin based mosquito coil on mice model. BMC Clin Pathol. 2017;17:19. doi: 10.1186/s12907-017-0057-9
Srinita G, Fuadi LN, Nazarudin N. PERBANDINGAN EFEKTIVITAS OBAT NYAMUK LISTRIK MAT YANG MENGANDUNG D-ALLETHRIN-TRANSFLUTHRIN DENGAN DIMEFLUTHRIN TERHADAP NYAMUK Aedes aegypti SEBAGAI VEKTOR DEMAM BERDARAH DENGUE. :1–10.
Andini A, Hasanah M, Azizah SN, Rosyadahan AR, Rimasari D, Triapadma W, et al. The Effect of Insect Repellent Exposure on Leukocyte Profile and Histopathologic Findings in Lungs. Biointerface Res Appl Chem. 2022;12(6):7796–803. doi: https://doi.org/10.33263/BRIAC126.77967803
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Niswah C, Syarifah, Sany F. PENGARUH ASAP ANTI NYAMUK KERTAS BAKAR TERHADAP JUMLAHERITROSIT PADA MENCIT ( Mus musculus L .). J SainHealth. 2018;2(1):40–5.
Armalina D, Witjahjo B, Susilaningsih N, Article O. Histopathological changes in liver, kidney and teratogenic effects of mice on exposure to mosquito repellent. J Kedokt dan Kesehat Indones. 2021;12(1):11–8. doi: 10.20885/JKKI.Vol12.Iss1.art4
Muhidin H, Rusmartini T. Perbandingan Efektivitas Obat Nyamuk Bakardan Elektrik dengan Bahan Aktif Dimeflutrin terhadap Nyamuk C ulex sp Mahasiswa Program Pendidikan Sarjana KedokteranUniversitas Islam Bandung , Dosen Fakultas Kedokteran Universitas Islam Bandung A . Pendahuluan. Pros Pendidik Dr. 2015;513–8.
Agata A, Widiastuti EL, Susanto GN. Respon Histopatologis Hepar Mencit ( Mus musculus ) yang Diinduksi Benzo ( α ) Piren terhadap Pemberian Taurin dan Ekstrak Daun Sirsak ( Annona muricata ). J Natur Indones. 2016;16(2):54–63.
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Laksana ASD, Kusumasita L, Faniyah F. Ameliorative effect of 50 % ethanol extract of moringa leaves ( Moringa oleifera Lam .) on lead-induced oxidative stress in the liver of male wistar rat model. Bali Med J. 2022;11(3):1887–91. doi: 10.15562/bmj.v11i3.3728
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[1] Al-Mamun MA, Ataur Rahman M, Habibur Rahman M, Hoque KMF, Ferdousi Z, Matin MN, et al. Biochemical and histological alterations induced by the smoke of allethrin based mosquito coil on mice model. BMC Clin Pathol. 2017;17:19. doi: 10.1186/s12907-017-0057-9

[2] Srinita G, Fuadi LN, Nazarudin N. PERBANDINGAN EFEKTIVITAS OBAT NYAMUK LISTRIK MAT YANG MENGANDUNG D-ALLETHRIN-TRANSFLUTHRIN DENGAN DIMEFLUTHRIN TERHADAP NYAMUK Aedes aegypti SEBAGAI VEKTOR DEMAM BERDARAH DENGUE. :1–10.

[3] Andini A, Hasanah M, Azizah SN, Rosyadahan AR, Rimasari D, Triapadma W, et al. The Effect of Insect Repellent Exposure on Leukocyte Profile and Histopathologic Findings in Lungs. Biointerface Res Appl Chem. 2022;12(6):7796–803. doi: https://doi.org/10.33263/BRIAC126.77967803

[4] Prihati DR, Nugraheni I. PENGARUH PAPARAN OBAT NYAMUK TERHADAP KADAR HEMOGLOBIN TIKUS BETINA USIA PUBERTAS Dwi Retna Prihati, Intan Nugraheni. J Terpadu Ilmu Kesehat. 2015;4(2):90–3.

[5] Niswah C, Syarifah, Sany F. PENGARUH ASAP ANTI NYAMUK KERTAS BAKAR TERHADAP JUMLAHERITROSIT PADA MENCIT ( Mus musculus L .). J SainHealth. 2018;2(1):40–5.

[6] Armalina D, Witjahjo B, Susilaningsih N, Article O. Histopathological changes in liver, kidney and teratogenic effects of mice on exposure to mosquito repellent. J Kedokt dan Kesehat Indones. 2021;12(1):11–8. doi: 10.20885/JKKI.Vol12.Iss1.art4

[7] Muhidin H, Rusmartini T. Perbandingan Efektivitas Obat Nyamuk Bakardan Elektrik dengan Bahan Aktif Dimeflutrin terhadap Nyamuk C ulex sp Mahasiswa Program Pendidikan Sarjana KedokteranUniversitas Islam Bandung , Dosen Fakultas Kedokteran Universitas Islam Bandung A . Pendahuluan. Pros Pendidik Dr. 2015;513–8.

[8] Agata A, Widiastuti EL, Susanto GN. Respon Histopatologis Hepar Mencit ( Mus musculus ) yang Diinduksi Benzo ( α ) Piren terhadap Pemberian Taurin dan Ekstrak Daun Sirsak ( Annona muricata ). J Natur Indones. 2016;16(2):54–63.

[9] Dahniar A. Pengaruh Asap Obat Nyamuk Terhadap Kesehatan dan Struktur Histologi Sistem Pernafasan. J Kedokt Syiah Kuala. 2011;11(1):52–9.

[10] Juhryyah SRI. Gambaran histopatologi organ hati dan ginjal tikus pada intoksikasi akut insektisida (metofluthrin, d-phenothrin, d-allethrin) dengan dosis bertingkat. 2008.

[11] Laksana ASD, Kusumasita L, Faniyah F. Ameliorative effect of 50 % ethanol extract of moringa leaves ( Moringa oleifera Lam .) on lead-induced oxidative stress in the liver of male wistar rat model. Bali Med J. 2022;11(3):1887–91. doi: 10.15562/bmj.v11i3.3728

[12] Sijid SA, Muthiadin C, Zulkarnain, Hidayat AS, Amelia RR. Pengaruh Pemberian Tuak terhadap Gambaran Histopatologi Hati Mencit (Mus musculus) ICR Jantan. J Pendidik Mat dan ipa. 2020;11(2):193–205. doi: dx.doi.org/10.26418/jpmipa.v11i2.36623

[13] Rinalty EDD, Soekanto A, Sahadewa S. Jus Buah Naga sebagai Antioksidan pada Tikus Putih (Rattus novergicus) yang Dipapar Obat Anti Nyamuk Bakar terhadap Perubahan Histopatologi Sel Nephron. J Ilm Pendidik Eksakta. 2017;3(3):235–49.

[14] Andreas H, Trianto HF, Ilmiawan MI. Gambaran Histologi Regenerasi Hati Pasca Penghentian Pajanan Monosodium Glutamat pada Tikus Wistar. eJournal Kedokt Indones. 2015;3(1):29–36. doi: 10.23886/ejki.3.4804.

[15] Suhita NLPR, Sudira IW, Winaya IBO. Histopatologi Ginjal Tikus Putih Akibat Pemberian Ekstrak Pegagan ( Centella asiatica ) Peroral. Bul Vet Udayana. 2013;5(2):71–8.

[16] Adinata MO, Sudira IW, Berata IK. Efek Ekstrak Daun Ashitaba (Angelica keiskei) Terhadap Gambaran Histopatologi Ginjal Mencit (Mus musculus) Jantan. Bul Vet Udayana. 2012;4(2):55–62.

Histopathological findings of the liver and kidneys of wistar rat exposed to insect repellent

Abstract

References

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