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Different clinicopathological characteristics in Indonesian colorectal patients with RAS mutations and LC3 over-expression: a cross-sectional study

  • Rio Andreas ,
  • Reno Rudiman ,
  • Kiki Lukman ,
  • Bambang Am Am Setya Sulthana ,
  • Andriana Purnama ,
  • Mochammad Riskie Aditya Putra ,
  • Prapanca Nugraha ,
  • Etis Primastari ,

Abstract

Link of Video Abstract: https://youtu.be/9Gip7ztxLJk

 

Background: Colorectal cancer is reaching the third highest incidence globally, including in Indonesia. Members of the RAS family are frequently discovered in colorectal cancers and may be a contributing factor for the successfulness of anti-epidermal growth factor receptor monoclonal antibodies therapy. Microtubule-associated protein 1 light chain 3 (LC3) is one of the autophagy markers in colorectal cancer cells routinely tested. The relevance of RAS mutation and expression of LC3 as a prognostic factor in CRC has so far only been discussed in several reports. This study aimed to describe the different clinicopathological characteristics of colorectal patients with mutations of the RAS family and LC3 overexpression.

Methods: This study was a cross-sectional study, and the subjects for this study were patient from the Digestive Surgery division at the Tertiary Hospital in West Java, Indonesia with colorectal cancer. A total of 40 study subjects were included in this study. Examination of RAS mutations was carried out by polymerase chain reaction. Meanwhile, the LC3 examination was performed with immunohistochemistry.

Results: The RAS family mutation, which is a mutation of either KRAS, NRAS, or both, was shown in 22 (55%) colorectal cancer patients, and a positive LC3 test was shown in 24 (60%) patients. A univariate analysis of the mutations of the RAS family and expression of the LC3 showed non-significant relationships.

Conclusion: No association observed between RAS family mutations and LC3 expression in colorectal cancer patients.

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How to Cite

Andreas, R., Rudiman, R., Lukman, K., Sulthana, B. A. A. S., Purnama, A., Putra, M. R. A., Nugraha, P., & Primastari, E. (2023). Different clinicopathological characteristics in Indonesian colorectal patients with RAS mutations and LC3 over-expression: a cross-sectional study. Bali Medical Journal, 12(2), 1774–1780. https://doi.org/10.15562/bmj.v12i2.4519

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Rio Andreas
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Reno Rudiman
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Kiki Lukman
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Bambang Am Am Setya Sulthana
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Andriana Purnama
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Mochammad Riskie Aditya Putra
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Prapanca Nugraha
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Etis Primastari
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