Comparison of Various Blood Vacuum Tubes Brand for Clinical Chemistry, Hormonal, Hematology, and Hemostasis Parameters

Astuti Giantini; Nuri Dyah  Indrasari; Siskawati Suparmin; Punjul Kasihanto; Arif Ramadhan  Tansir

doi:10.15562/bmj.v12i3.4685

Comparison of Various Blood Vacuum Tubes Brand for Clinical Chemistry, Hormonal, Hematology, and Hemostasis Parameters

Astuti Giantini ,

Nuri Dyah Indrasari ,

Siskawati Suparmin ,

Punjul Kasihanto ,

Arif Ramadhan Tansir ,

pdf |
DOI: https://doi.org/10.15562/bmj.v12i3.4685 |
Published: 2023-10-07

Abstract

Link of Video Abstract: https://youtu.be/O7-fCHyvGo4

Background: Laboratory tests are integral to the medical decision-making process. Blood vacuum tubes need to be validated to increase the analytics quality. This study aimed to compare various vacuum tube brands that use serum clot activator and serum separator tube (SST) for clinical chemistry and hormonal parameters, K3EDTA anticoagulant for hematological parameters, and citrate for hemostasis parameters.

Methods: The comparative study was carried out cross-sectional in the Department of Clinical Pathology, Dr. Cipto Mangunkusumo National Public Hospital, on June 20, 2020. There were 20 subjects for each type of tube. The control tube was BD Vacutainer®, and the test tubes were Samplix®, VACUETTE®, Vacusera®, and YCHD.

Results: Underfilling was the most common problem in preanalytical observation. This problem for citrate tubes was most frequently found in Vacusera® tubes. The possible cause of underfilling is the expiration date, which was close to the date of the experiment. There was a significant difference in PT results (p <0.0001) in the Vacusera® citrate tube. All tube brands had no significant differences in all clinical chemistry, hormonal, and hematological parameters (P>0.05). After 24 hours at room temperature, the results of hematological parameters showed statistically significant differences in MCV and hematocrit parameters, while other parameters were still within the CV limit of the instrument.

Conclusion: There were no significant differences in the results of clinical chemistry, hormonal, and hematological parameters for all tube brands. A hematology parameters test should be done immediately as the delay may affect the MCV and hematocrit parameters.

References

Lippi G, Cornes M, Granckvist K, Nybo M, Simundic A. EFLM WG-Preanalytical phase opinion paper: local validation of blood collection tubes in clinical laboratories. Clin Chem Lab Med. 2016;54(5):755-760.
Bowen RAR, Remaley AT. Interferences from blood collection tube components on clinical chemistry assays. Biochem Med. 2014;24(1):31-44.
Clinical and Laboratory Standards Institute (CLSI). Method Comparison and Bias Estimation Using Patient Samples; Approved Guideline. CLSI document EP9-A. Wayne, PA: Clinical Laboratory Standards Institute; 1992.
Karakoyun I, Arslan FD, Onur S, Ozturk YK, Parildar H, Basok BI. Comparison of 30 biochemical analytes in 3 different blood collection tubes. Türk Klinik Biyokimya Derg 2020;18(2):61-69.
Lima-Oliveira G, Lippi G, Salvagno G, Montagnana M, Picheth G, Guidi G. Preanalytical management: serum vacuum tubes validation for routine clinical chemistry. Biochem Medica. 2012;22(2):180-186.
Sysmex Corporation. Automated hematology analyzer XN series (For XN-2000 system) instruction for use. Kobe: Sysmex Corporation, 2011;1(1):2-8.
Kitchen S, Adcock DM, Dauer R, Kristoffersen AH, Lippi G, Mackie I, et al. International Council for Standardization in Haematology (ICSH) recommendations for processing of blood samples for coagulation testing. Int J Lab Hematol. 2021;43(6):1272-1283.
Zou J, Nolan D, LaFiore A, Scott M. Estimating the effects of hemolysis on potassium and LDH laboratory results. Clin Chim Acta. 2013;421(1):60-61.
Dewi MMW, Herawati S, Mulyantari NK, Prabawa IPY. The comparison of erythrocyte sedimentation rate (ESR) modify Westergren Caretium Xc-A30 and Westergren Manual in Clinical Pathology Laboratory, Sanglah General Hospital, Denpasar, Bali. Bali Medical Journal. 2019;8(2):487-490.
Zulkafli Z, Mustaffa R, Yusoff ASM. Hematopoietic Progenitor Cells as a Predictive of Cd34+ Enumeration Prior to Peripheral Blood Stem Cells Harvesting. Bali Medical Journal. 2014;3(3):112–115.
Harahap RIM, Prihatni D, Rostini T. The compatibility measurement of Mentzer, England & Fraser, Shine & Lal, and Srivastava indices to the hemoglobin electrophoresis result for beta thalassemia trait screening. Bali Medical Journal. 2019;8(2):403–407.
Rahmanitarini A, Hernaningsih Y, Indrasari YN. The stability of sample storage for complete blood count (CBC) toward the blood cell morphology. Bali Medical Journal. 2019;8(2):482–486.

[1] Lippi G, Cornes M, Granckvist K, Nybo M, Simundic A. EFLM WG-Preanalytical phase opinion paper: local validation of blood collection tubes in clinical laboratories. Clin Chem Lab Med. 2016;54(5):755-760.

[2] Bowen RAR, Remaley AT. Interferences from blood collection tube components on clinical chemistry assays. Biochem Med. 2014;24(1):31-44.

[3] Clinical and Laboratory Standards Institute (CLSI). Method Comparison and Bias Estimation Using Patient Samples; Approved Guideline. CLSI document EP9-A. Wayne, PA: Clinical Laboratory Standards Institute; 1992.

[4] Karakoyun I, Arslan FD, Onur S, Ozturk YK, Parildar H, Basok BI. Comparison of 30 biochemical analytes in 3 different blood collection tubes. Türk Klinik Biyokimya Derg 2020;18(2):61-69.

[5] Lima-Oliveira G, Lippi G, Salvagno G, Montagnana M, Picheth G, Guidi G. Preanalytical management: serum vacuum tubes validation for routine clinical chemistry. Biochem Medica. 2012;22(2):180-186.

[6] Sysmex Corporation. Automated hematology analyzer XN series (For XN-2000 system) instruction for use. Kobe: Sysmex Corporation, 2011;1(1):2-8.

[7] Kitchen S, Adcock DM, Dauer R, Kristoffersen AH, Lippi G, Mackie I, et al. International Council for Standardization in Haematology (ICSH) recommendations for processing of blood samples for coagulation testing. Int J Lab Hematol. 2021;43(6):1272-1283.

[8] Zou J, Nolan D, LaFiore A, Scott M. Estimating the effects of hemolysis on potassium and LDH laboratory results. Clin Chim Acta. 2013;421(1):60-61.

[9] Dewi MMW, Herawati S, Mulyantari NK, Prabawa IPY. The comparison of erythrocyte sedimentation rate (ESR) modify Westergren Caretium Xc-A30 and Westergren Manual in Clinical Pathology Laboratory, Sanglah General Hospital, Denpasar, Bali. Bali Medical Journal. 2019;8(2):487-490.

[10] Zulkafli Z, Mustaffa R, Yusoff ASM. Hematopoietic Progenitor Cells as a Predictive of Cd34+ Enumeration Prior to Peripheral Blood Stem Cells Harvesting. Bali Medical Journal. 2014;3(3):112–115.

[11] Harahap RIM, Prihatni D, Rostini T. The compatibility measurement of Mentzer, England & Fraser, Shine & Lal, and Srivastava indices to the hemoglobin electrophoresis result for beta thalassemia trait screening. Bali Medical Journal. 2019;8(2):403–407.

[12] Rahmanitarini A, Hernaningsih Y, Indrasari YN. The stability of sample storage for complete blood count (CBC) toward the blood cell morphology. Bali Medical Journal. 2019;8(2):482–486.

Comparison of Various Blood Vacuum Tubes Brand for Clinical Chemistry, Hormonal, Hematology, and Hemostasis Parameters

Abstract

References

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