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Propolis effects on dysbiosis of polycystic ovarian syndrome (PCOS) induced Wistar rats

Abstract

Introduction: Polycystic ovarian syndrome (PCOS) is an endocrinopathy that has two major risk factors, genetics and environment. Tremellen and Pearce proposed a new theory of Dysbiosis of Gut Microbiota (DOGMA) as an environmental factor that can modified by probiotics. Propolis acts as a probiotic in diabetic rats and has been proven to change gut microbiota dysbiosis. This study evaluated whether propolis extract can modify dysbiosis in PCOS animal models compared to metformin.

Methods: This was a post-test-only control group design randomized control trial with 30 Wistar rats divided into five groups: control, PCOS, PCOS with metformin, PCOS with propolis extract, and PCOS with metformin plus propolis extract. PCOS induced by daily 100 mg/kg.bw testosterone propionate injection intramuscularly for 28 days. The feces in the caecum were extracted after the rats were sacrificed and analyzed for Firmicutes/Bacteroides ratio, alpha diversity, and beta diversity.

Result: Firmicutes abundance in the control group was the lowest, and Bacteroidetes abundance was lowest in the propolis group, although it’s not significantly different. Between groups, Firmicutes/Bacteroidetes ratio, alpha diversity, and beta diversity were not significantly different. Testosterone concentration and preantral follicle count in the propolis and metformin groups improved compared to the PCOS group (p=0,01 and p=0,025, respectively).

Conclusion: Propolis cannot modify dysbiosis in PCOS-induced rats, but it reduces total testosterone concentration and preantral follicle counts.

References

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How to Cite

Puspitasari, V. D., Lestari, E. S., & Pramono, N. (2024). Propolis effects on dysbiosis of polycystic ovarian syndrome (PCOS) induced Wistar rats . Bali Medical Journal, 13(1), 924–929. https://doi.org/10.15562/bmj.v13i1.5147

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Vannya Dewi Puspitasari
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Endang Sri Lestari
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Noor Pramono
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