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The anti-inflammatory potential of epigallocatechin gallate (EGCG) on tumor necrosis factor alpha and interleukin–10 expression in pseudomonas keratitis model (in vivo study on Rattus norvegicus rats model)

  • Fariztah Sukainah Nur Fathimah ,
  • Ismi Zuhria ,
  • Delfitri Lutfi ,
  • Tarosa Yodia Urolita ,
  • Annisa Karima ,
  • Nila Kurniasari ,
  • Djoko Agus Purwanto ,


Background: Pseudomonas aeruginosa infection is the primary cause of keratitis, leading to gradual corneal deterioration and resulting in scarring, thinning, and corneal perforation. Antibiotic treatment alone may prove ineffectual in certain instances, as the inflammatory process may remain despite the elimination of bacteria. Due to its antibacterial, anti-inflammatory, and antioxidant properties, Epigallocatechin gallate (EGCG) has the potential to be used as an adjuvant therapy for Pseudomonas keratitis. The objective of this study is to analyze the expression of TNF-α and IL-10 in a P. aeruginosa-induced keratitis model following the administration of moxifloxacin and EGCG eye drops.

Methods: Rattus norvegicus with keratitis induced by clinical isolate P. aeruginosa were randomly allocated to one of three treatment groups: the negative control group given NaCl 0.93%  and benzalkonium chloride 0.01%; the group given moxifloxacin eye drops; and the group given moxifloxacin eye drops and EGCG 50 μg/mL on the third to fifth day after keratitis induction. Immunohistochemical staining of corneal tissue was utilized to analyze the levels of TNF-α and IL-10 expression.

Results: On the fifth day of subsequent keratitis induction, a statistically significant difference in TNF-α expression was observed across the groups (p=0.015). It was found between the moxifloxacin and EGCG 50 g/mL groups and the negative control group (p=0.008). IL-10 expression showed no significant difference across the groups (p=0.108).

Conclusion: TNF-α expression was significantly different among the three groups, whereas IL-10 expression was not significantly different.


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How to Cite

Fathimah, F. S. N. ., Zuhria, I. ., Lutfi, D. ., Urolita, T. Y. ., Karima, A. ., Kurniasari, N. ., & Purwanto, D. A. . (2024). The anti-inflammatory potential of epigallocatechin gallate (EGCG) on tumor necrosis factor alpha and interleukin–10 expression in pseudomonas keratitis model (in vivo study on Rattus norvegicus rats model). Bali Medical Journal, 13(2), 551–557.




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Fariztah Sukainah Nur Fathimah
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BMJ Journal

Ismi Zuhria
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Delfitri Lutfi
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Tarosa Yodia Urolita
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Annisa Karima
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Nila Kurniasari
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Djoko Agus Purwanto
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