ORIGINAL ARTICLE

Assesing sensitivity and specificity of rapid diagnostic test : the importance and challenges of Influenza surveillance in Indonesia

Vivi Setiawaty , Roselinda Roselinda, Subangkit Subangkit, Krisna Nur Andriana Pangesti

Vivi Setiawaty
National Institute of Health Research and Development / Badan Litbang Kesehatan. Email: vivisetiawaty@hotmail.com

Roselinda Roselinda
National Insitute of Health Research and Development, Jakarta, Indonesia

Subangkit Subangkit
National Insitute of Health Research and Development, Jakarta, Indonesia

Krisna Nur Andriana Pangesti

Online First: July 19, 2017 | Cite this Article
Setiawaty, V., Roselinda, R., Subangkit, S., Pangesti, K. 2017. Assesing sensitivity and specificity of rapid diagnostic test : the importance and challenges of Influenza surveillance in Indonesia. Bali Medical Journal 6(3): 500-504. DOI:10.15562/bmj.v6i3.570


Background: The rapid detection of influenza viruses from clinical samples is important for establishing public health  and clinical decisions, including infection prevention and control measures. The SD Bioline rapid influenza test (Standard Diagnostics, Inc., Suwon, Korea) is a point-of-care (POC) diagnostic test which based on influenza-specific monoclonal antibodies. The purpose of this study is to assess the performance of this assay against the reference diagnostic standards of RT-PCR for Influenza diagnosis in Surveillance activity.

Methods: 4,262 nasal and throat swab specimens were collected from influenza-like illness (ILI) patients from sentinel hospital and health-care sites across 10 provinces in Indonesia. Specimens were tested with the SD Bioline rapid influenza test in sentinels and rRT-PCR test was conducted in referral laboratory in Jakarta. Data analysis was performed by comparing the results of rapid test conducted in the POC settting and the results of  rRT-PCR test.

Results: Testing of throat and nasal swabs from children and adults indicated the specificity of The SD Bioline rapid influenza test to be 97% for influenza A and B, respectively, in 4262 specimens. However, the overall sensitivity of the SD Bioline rapid influenza test  for these samples was  low at 25%.

Conclusions: Overall, the results indicated that the SD Bioline rapid influenza test has a high specificity but low sensitivity for detection influenza from throat and nasal swabs therefore it will be a challenge of laboratory testing on site.

Keywords: Influenza, rapid diagnostic test, surveillance

References

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