ORIGINAL ARTICLE

The difference in motor improvements related to combination of omega-3 polyunsaturated fatty acid and alpha-tocopherol supplementations diet of weight-dropped induced spinal cord injury in rats

Tjokorda GB Mahadewa , Wisnu Arya Wardana, Wisnu Wardhana

Tjokorda GB Mahadewa
Neurosurgery Department Udayana University-Sanglah Hospital, Bali, Indonesia. Email: tjokmahadewa@unud.ac.id

Wisnu Arya Wardana
Neurosurgery Department Udayana University-Sanglah Hospital, Bali, Indonesia

Wisnu Wardhana
Neurosurgery Department Udayana University-Sanglah Hospital, Bali, Indonesia
Online First: August 04, 2017 | Cite this Article
Mahadewa, T., Wardana, W., Wardhana, W. 2017. The difference in motor improvements related to combination of omega-3 polyunsaturated fatty acid and alpha-tocopherol supplementations diet of weight-dropped induced spinal cord injury in rats. Bali Medical Journal 6(3): S22-S25. DOI:10.15562/bmj.v6i3.711


Background: Spinal cord injury (SCI) is the cause of severe neurological disability, and no satisfactory treatment is currently available. Evidence suggests that omega-3 polyunsaturated fatty acid (PUFA)  could target some of the pathological mechanisms that underlie damage after SCI. In parallel, promising effect of alpha-tocopherol (vitamin E) also shown as a potent antioxidant to prevent lipid peroxidation on SCI. Therefore, we aim to investigate the combined effect of omega-3 PUFA and vitamin E supplementation in improving motor function of weight dropped induced SCI in rats. Methods: Twenty-five rats randomly divided into the five groups. SCI was induced in the rats by using a weight dropped model around T9-T11 vertebra level. Omega-3 PUFA and vitamin E was administered orally on each day for two weeks of treatment. The sham group underwent laminectomy without spinal cord compression and treatment. Motor function was measured at the 1st, 7th and 14th day. Results: We found the significant difference of motoric function between omega-3 PUFA group and combination treatment group compared to the control (p<0,05). The highest BBB score on the first week was found in combination treatment group (mean 16±2.3), but no significant difference was found compared to the omega-3 PUFA group. On the subsequent week, combination treatment was showing marked improvement compared to the omega-3 PUFA group (p<0,05). Conclusion: These finding had demonstrated the effect of Omega-3 PUFA as a neuroprotective agent to prevent the secondary injury in SCI-induced rats. The combination of omega-3 PUFA with vitamin E improves its efficacy to become a promising novel therapy for SCI.

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