ORIGINAL ARTICLE

Quantification analysis of tissue eosinophilia in squamous cell carcinoma of the head and neck region

M Sabzijate , AH Khatibi, T Ghiasian, S Rahrotaban, E Rastegar, SH Eftekharian

M Sabzijate
Orthodontics resident, Shahed University of Medical Sciences, Iran. Email: msabzijate@gmail.com

AH Khatibi
Orthodontics resident, Shahed University of Medical Sciences, Iran

T Ghiasian
dentist, Private practitioner, Tehran, Iran

S Rahrotaban
Assistant professor of oral and maxillofacial pathology dentistry, Tehran University of Medical Sciences, Tehran, Iran

E Rastegar
Orthodontics resident, Shahed University of Medical Sciences, Iran

SH Eftekharian
Orthodontics resident, Shahed University of Medical Sciences, Iran
Online First: April 15, 2018 | Cite this Article
Sabzijate, M., Khatibi, A., Ghiasian, T., Rahrotaban, S., Rastegar, E., Eftekharian, S. 2018. Quantification analysis of tissue eosinophilia in squamous cell carcinoma of the head and neck region. Bali Medical Journal 7(1): 165-169. DOI:10.15562/bmj.v7i1.787


Background and aim: Tumor-associated tissue eosinophilia (TATE) is commonly present in different neoplasms, yet its relationship with cancers has not been established. Squamous cell carcinoma (SCC) is one of the most common malignancies of the head and neck region. The aim of this study was to evaluate the relationship between TATE and histological grading of head and neck SCC (HNSCC) using Luna staining technique.

Materials and methods: In the present descriptive study, 67 samples consisting of 4-µm cross-sections of HNSCC tissue stained with hematoxylin and eosin (H&E) were re-evaluated for histological grading. The samples were divided into three categories of well differentiated, moderately differentiated, and poorly differentiated. Luna histochemical staining was used to evaluate infiltration of eosinophils in the tumor. Eosinophils were randomly counted in 10 microscopic fields and divided into 4 categories. Kruskal-Wallis test and Spearman's correlation coefficient were used to evaluate the relationship between eosinophilic infiltration and other relevant factors.

Results: The frequency of tissue eosinophilia was zero in only one case (1.5%) and was positive in the rest 66 cases (98.5%). The mean count of eosinophils was 7.5±4.5 cells per high-power field (HPF). There was a significant relationship between histopathological grading and TATE (p = 0.04). However, there were no significant relationships between TATE and factors such as age, gender, and lesion location.

Conclusion: The tissue eosinophil counts in the group with poorly differentiated SCC of the head and neck region were less than those in the two other groups; regardless, no significant relationship was found with other clinicopathological factors.

 

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