Association between expression of ALDH1 and TGFb2 genes, histological and clinical findings in breast cancer

Yasan Sadeghian, Esmaeil Samizadeh, Mohammad Ali Ali Mohammadi-vajari, Mohammad Hossein Lashkari , Ehsan Sadeghian, Seyedeh Nasim Hosseini, Mehdi jafari, Tahereh Sorbi

Yasan Sadeghian
General Physician, School of Medicine, Shahid Beheshti University of Medical Sciences AND Medical Researcher, AJA University of Medical Sciences, Tehran, Iran

Esmaeil Samizadeh
Pathologist, Department of Pathology, School of Medicine, Imam Reza Hospital, AJA University of Medical Sciences, Tehran, Iran

Mohammad Ali Ali Mohammadi-vajari
General physician, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Mohammad Hossein Lashkari
Professor of Surgery, School of Medicine, AJA University of Medical Sciences, Tehran, Iran. Email: mhlashkari8@gmail.com

Ehsan Sadeghian
Resident, Department of General Surgery, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Seyedeh Nasim Hosseini
Biotechnologist, national institute of genetic engineering and biotechnology (NIGEB), Tehran, Iran

Mehdi jafari
Epidemiologist, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Tahereh Sorbi
Anesthesiologist, Department of Anesthesiology, School of Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
Online First: December 01, 2019 | Cite this Article
Sadeghian, Y., Samizadeh, E., Mohammadi-vajari, M., Lashkari, M., Sadeghian, E., Hosseini, S., jafari, M., Sorbi, T. 2019. Association between expression of ALDH1 and TGFb2 genes, histological and clinical findings in breast cancer. Bali Medical Journal 8(3): 813-817. DOI:10.15562/bmj.v8i3.835

Background: Breast cancer is one of the most common malignancies. The relationship between the pathogenesis of breast tumours and their invasive behaviour is an important issue that has been discussed recently. ALDH1 and TGFb2 genes can be mentioned among the most crucial signalling and molecular pathways that play an active role in tumour invasive behaviour. The aim of this study was to determine the expression level of ALDH1 and TGFb2 genes in breast cancer and its relationship with histology in breast cancer patients.

Method and material: This cross-sectional study was done on 65 breast cancer patients who had been referred to the Army 501 hospital during the years of 1992-1994. Data was collected using the patient records, and the results of immunohistochemically staining were obtained in the laboratory. Data were analyzed by SPSS software using t-test, Chi-square and Fisher tests. The level of significance was considered to be 0.05.

Results: In this study, we assessed the expression of these genes in tissue samples of 65 patients with breast cancer and its relation with the histology and clinical progress. The expression of ALDH1 in the malignant breast tissue was 83.5% (55 out of 65 samples) and the expression of TGFb2 gene showed 54 samples (83.1%) were positive. There was a significant relationship between lymphatic drainage and ALDH1 expression.

Conclusion: There was a significant relationship between ALDH1 expression, tumour size, neuronal invasion, tumour grade, metastasis, and lymph node involvement. There was also a significant relationship between TGFb2 expression and tumour size, metastasis, tumour grade, and lymph node involvement. There was no significant relationship between the expression of ALDH1, TGFb2, and the type of pathology and history of chemotherapy


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